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Recruitment of Cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets

Wang, Hongyin, Carey, Lucas B., Cai, Ying, Wijnen, Herman and Futcher, Bruce (2009) Recruitment of Cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets PLoS Biology, 7, (9), e1000189-[14pp]. (doi:10.1371/journal.pbio.1000189). (PMID:19823669).

Record type: Article


In yeast, the G1 cyclin Cln3 promotes cell cycle entry by activating the transcription factor SBF. In mammals, there is a parallel system for cell cycle entry in which cyclin dependent kinase (CDK) activates transcription factor E2F/Dp. Here we show that Cln3 regulates SBF by at least two different pathways, one involving the repressive protein Whi5, and the second involving Stb1. The Rpd3 histone deacetylase complex is also involved. Cln3 binds to SBF at the CLN2 promoter, and removes previously bound Whi5 and histone deacetylase. Adding extra copies of the SBF binding site to the cell delays Start, possibly by titrating Cln3. Since Rpd3 is the yeast ortholog of mammalian HDAC1, there is now a virtually complete analogy between the proteins regulating cell cycle entry in yeast (SBF, Cln3, Whi5 and Stb1, Rpd3) and mammals (E2F, Cyclin D, Rb, HDAC1). The cell may titrate Cln3 molecules against the number of SBF binding sites, and this could be the underlying basis of the size-control mechanism for Start.

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e-pub ahead of print date: 8 September 2009
Published date: September 2009
Organisations: Centre for Biological Sciences


Local EPrints ID: 340369
ISSN: 1544-9173
PURE UUID: 8bbb9115-57fb-455e-8bc0-13dcfe857b4b

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Date deposited: 19 Jun 2012 15:53
Last modified: 18 Jul 2017 05:45

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Author: Hongyin Wang
Author: Lucas B. Carey
Author: Ying Cai
Author: Herman Wijnen
Author: Bruce Futcher

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