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MMP-1 drives immunopathology in human tuberculosis and transgenic mice

Elkington, Paul, Shiomi, Takayuki, Breen, Ronan, Nuttall, Robert K., Ugarte-Gil, Cesar Augusto, Walker, Naomi F., Saraiva, Luísa, Pedersen, Bernadette, Mauri, Francesco, Lipman, Marc, Edwards, Dylan R., Robertson, Brian D., D’Armiento, Jeanine and Friedland, Jon S. (2011) MMP-1 drives immunopathology in human tuberculosis and transgenic mice Journal of Clinical Investigation, 121, (5), pp. 1827-1833. (doi:10.1172/JCI45666).

Record type: Article


Mycobacterium tuberculosis can cause lung tissue damage to spread, but the mechanisms driving this immunopathology are poorly understood. The breakdown of lung matrix involves MMPs, which have a unique ability to degrade fibrillar collagens at neutral pH. To determine whether MMPs play a role in the immunopathology of tuberculosis (TB), we profiled MMPs and their inhibitors, the tissue inhibitor of metalloproteinases (TIMPs), in sputum and bronchoalveolar lavage fluid from patients with TB and symptomatic controls. MMP-1 concentrations were significantly increased in both HIV-negative and HIV-positive patients with TB, while TIMP concentrations were lower in HIV-negative TB patients. In primary human monocytes, M. tuberculosis infection selectively upregulated MMP1 gene expression and secretion, and Ro32-3555, a specific MMP inhibitor, suppressed M. tuberculosis–driven MMP-1 activity. Since the mouse MMP-1 ortholog is not expressed in the lung and mice infected with M. tuberculosis do not develop tissue destruction equivalent to humans, we infected transgenic mice expressing human MMP-1 with M. tuberculosis to investigate whether MMP-1 caused lung immunopathology. In the MMP-1 transgenic mice, M. tuberculosis infection increased MMP-1 expression, resulting in alveolar destruction in lung granulomas and significantly greater collagen breakdown. In summary, MMP-1 may drive tissue destruction in TB and represents a therapeutic target to limit immunopathology.

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Published date: May 2011
Organisations: Faculty of Medicine


Local EPrints ID: 340684
ISSN: 0021-9738
PURE UUID: 6d0b5441-f7aa-4f42-a2cd-56e718e291c2
ORCID for Paul Elkington: ORCID iD

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Date deposited: 28 Jun 2012 15:45
Last modified: 18 Jul 2017 05:42

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Author: Paul Elkington ORCID iD
Author: Takayuki Shiomi
Author: Ronan Breen
Author: Robert K. Nuttall
Author: Cesar Augusto Ugarte-Gil
Author: Naomi F. Walker
Author: Luísa Saraiva
Author: Bernadette Pedersen
Author: Francesco Mauri
Author: Marc Lipman
Author: Dylan R. Edwards
Author: Brian D. Robertson
Author: Jeanine D’Armiento
Author: Jon S. Friedland

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