Analysis of matrix metalloproteinase secretion by macrophages
Analysis of matrix metalloproteinase secretion by macrophages
Matrix metalloproteinases (MMPs) are zinc-dependent proteases whose physiological roles include control of leukocyte migration. They are implicated in tissue destruction in inflammatory and infectious diseases. MMPs are not only capable of degrading all components of the extracellular matrix, but they also can modulate the immune response by cleaving cytokines and chemokines to alter their activity. Macrophages secrete a broad range of MMPs and represent a key source of MMPs in inflammatory lesions such as granulomas. Zymography is substrate-based gel electrophoresis that allows direct visualization of MMP activity. Here we describe measurement of MMP secretion from macrophages focusing on quantitative zymography. We also discuss complementary methods that should be used in parallel with zymography. The ability to analyze and quantify MMP secretion by macrophages offers an additional window through which to understand the contributions of macrophages to a wide variety of infectious, inflammatory, and immunologic disorders.
253-265
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Green, Justin A.
9c179973-04b4-48cb-a685-1078e3ab13cd
Friedland, Jon S.
9968669f-afe0-4163-9b35-3b476246fd4a
2009
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Green, Justin A.
9c179973-04b4-48cb-a685-1078e3ab13cd
Friedland, Jon S.
9968669f-afe0-4163-9b35-3b476246fd4a
Abstract
Matrix metalloproteinases (MMPs) are zinc-dependent proteases whose physiological roles include control of leukocyte migration. They are implicated in tissue destruction in inflammatory and infectious diseases. MMPs are not only capable of degrading all components of the extracellular matrix, but they also can modulate the immune response by cleaving cytokines and chemokines to alter their activity. Macrophages secrete a broad range of MMPs and represent a key source of MMPs in inflammatory lesions such as granulomas. Zymography is substrate-based gel electrophoresis that allows direct visualization of MMP activity. Here we describe measurement of MMP secretion from macrophages focusing on quantitative zymography. We also discuss complementary methods that should be used in parallel with zymography. The ability to analyze and quantify MMP secretion by macrophages offers an additional window through which to understand the contributions of macrophages to a wide variety of infectious, inflammatory, and immunologic disorders.
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Published date: 2009
Organisations:
Faculty of Medicine
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Local EPrints ID: 341056
URI: http://eprints.soton.ac.uk/id/eprint/341056
PURE UUID: 19fec57e-ddbe-4401-91bf-aa801912e0b0
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Date deposited: 12 Jul 2012 09:30
Last modified: 15 Mar 2024 03:43
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Author:
Justin A. Green
Author:
Jon S. Friedland
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