The University of Southampton
University of Southampton Institutional Repository

Analysis of matrix metalloproteinase secretion by macrophages

Elkington, Paul T., Green, Justin A. and Friedland, Jon S. (2009) Analysis of matrix metalloproteinase secretion by macrophages Methods in Molecular Biology, 531, pp. 253-265. (doi:10.1007/978-1-59745-396-7_16). (PMID:19347322).

Record type: Article

Abstract

Matrix metalloproteinases (MMPs) are zinc-dependent proteases whose physiological roles include control of leukocyte migration. They are implicated in tissue destruction in inflammatory and infectious diseases. MMPs are not only capable of degrading all components of the extracellular matrix, but they also can modulate the immune response by cleaving cytokines and chemokines to alter their activity. Macrophages secrete a broad range of MMPs and represent a key source of MMPs in inflammatory lesions such as granulomas. Zymography is substrate-based gel electrophoresis that allows direct visualization of MMP activity. Here we describe measurement of MMP secretion from macrophages focusing on quantitative zymography. We also discuss complementary methods that should be used in parallel with zymography. The ability to analyze and quantify MMP secretion by macrophages offers an additional window through which to understand the contributions of macrophages to a wide variety of infectious, inflammatory, and immunologic disorders.

Full text not available from this repository.

More information

Published date: 2009
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 341056
URI: http://eprints.soton.ac.uk/id/eprint/341056
PURE UUID: 19fec57e-ddbe-4401-91bf-aa801912e0b0
ORCID for Paul T. Elkington: ORCID iD orcid.org/0000-0003-0390-0613

Catalogue record

Date deposited: 12 Jul 2012 09:30
Last modified: 18 Jul 2017 05:39

Export record

Altmetrics

Contributors

Author: Justin A. Green
Author: Jon S. Friedland

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×