The University of Southampton
University of Southampton Institutional Repository

Compartmentalization of electrophoretically separated analytes in a multiphase microfluidic platform

Compartmentalization of electrophoretically separated analytes in a multiphase microfluidic platform
Compartmentalization of electrophoretically separated analytes in a multiphase microfluidic platform
Herein, we describe the monolithic integration of a multiphase microfluidic system to a microcapillary gel electrophoresis (?CGE) architecture for the complete isolation and storage of separated analyte bands. Within this platform, analyte molecules are separated using microchannel gel electrophoresis, and the eluted bands are stored in a sequence of approximately 40?600 encapsulating microdroplets. Importantly, employing such a system allows for total control of droplet size, shape, and composition. This approach is utilized to separate, optically detect, and encapsulate two fluorescent analytes from a composite sample mixture. Further to this, we subsequently investigate the potential of the system to be used as a concentration gradient generator through analysis of the segmented analyte bands and droplet composition.
0003-2700
5801-5808
Draper, Mark C.
f989933d-b9d2-4ad8-aa05-691abf76b8e2
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
Cho, Soongwon
0afaae85-b6f1-463f-bbcb-a81361c26de5
James, David I.
e4f78d0e-35d6-4667-a0bc-154ccdfda9d2
Edel, Joshua B.
8397afdd-a0dc-489b-83e8-58a75ca46732
Draper, Mark C.
f989933d-b9d2-4ad8-aa05-691abf76b8e2
Niu, Xize
f3d964fb-23b4-45db-92fe-02426e4e76fa
Cho, Soongwon
0afaae85-b6f1-463f-bbcb-a81361c26de5
James, David I.
e4f78d0e-35d6-4667-a0bc-154ccdfda9d2
Edel, Joshua B.
8397afdd-a0dc-489b-83e8-58a75ca46732

Draper, Mark C., Niu, Xize, Cho, Soongwon, James, David I. and Edel, Joshua B. (2012) Compartmentalization of electrophoretically separated analytes in a multiphase microfluidic platform. Analytical Chemistry, 84 (13), 5801-5808. (doi:10.1021/ac301141x).

Record type: Article

Abstract

Herein, we describe the monolithic integration of a multiphase microfluidic system to a microcapillary gel electrophoresis (?CGE) architecture for the complete isolation and storage of separated analyte bands. Within this platform, analyte molecules are separated using microchannel gel electrophoresis, and the eluted bands are stored in a sequence of approximately 40?600 encapsulating microdroplets. Importantly, employing such a system allows for total control of droplet size, shape, and composition. This approach is utilized to separate, optically detect, and encapsulate two fluorescent analytes from a composite sample mixture. Further to this, we subsequently investigate the potential of the system to be used as a concentration gradient generator through analysis of the segmented analyte bands and droplet composition.

This record has no associated files available for download.

More information

e-pub ahead of print date: 13 June 2012
Published date: 3 July 2012
Organisations: Mechatronics

Identifiers

Local EPrints ID: 341132
URI: http://eprints.soton.ac.uk/id/eprint/341132
ISSN: 0003-2700
PURE UUID: 5478328a-b070-4bf1-b38a-7a14c09eb240

Catalogue record

Date deposited: 16 Jul 2012 10:52
Last modified: 14 Mar 2024 11:35

Export record

Altmetrics

Contributors

Author: Mark C. Draper
Author: Xize Niu
Author: Soongwon Cho
Author: David I. James
Author: Joshua B. Edel

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×