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Oral administration of oleic or linoleic acids modulates the production of inflammatory mediators by rat macrophages

Oral administration of oleic or linoleic acids modulates the production of inflammatory mediators by rat macrophages
Oral administration of oleic or linoleic acids modulates the production of inflammatory mediators by rat macrophages
Oleic (OLA) and linoleic (LNA) acids are commonly consumed fatty acids and they can modulate the inflammatory response, in which macrophages play an important role. The aim of this study was to investigate the effects of these two fatty acids on the production of inflammatory mediators by macrophages. Rats received oral administration of water (control), OLA or LNA(0.22 g/kg body weight) daily for 10 days and peritoneal resident macrophages were then isolated. Subsequently, they were seeded in culture plates and the production of various inflammatory mediators was assessed. Oral administration with OLA decreased the production of IL-1b, IL-6 and CINC-2ab by resident macrophages and LNA decreased the production of IL-1b, IL-6 and VEGF in the absence of lipopolysaccharide (LPS), although it accelerated IL-1b release and decreased IL-10 synthesis when cells were stimulated with LPS. Neither fatty acid affected the production of superoxide anion, hydrogen peroxide, nitrite, TNF-a, PGE2, LTB4 or 15(S)-HETE.
Thus, OLA and LNA influence the production of several
inflammatory mediators by macrophages.
Fatty acids, inflammation, ROS, RNS, cytokines, VEGF, Eicosanoids
0024-4201
803-812
Magdalon, Juliana
27683b0c-d552-4276-bebf-94ba21d67071
Vinolo, Mario A. R.
36472b53-5030-4ca6-bbb5-0918fb767011
Rodrigues, Hosana G.
6bfd2f76-eeb9-44af-b3da-ab19b8f0d1e1
Paschoal, Vivian A.
bcf93f87-e7c7-4f73-be3d-64493e988687
Torres, Rosângela P.
cd91fc72-b6e2-48f7-889f-bf312472d992
Mancini-Filho, Jorge
0b3bccee-44a5-4695-bfbb-5f6a317bcfbc
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Hatanaka, Elaine
a75419b0-65c9-496b-ba2e-62e716fd0965
Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec
Magdalon, Juliana
27683b0c-d552-4276-bebf-94ba21d67071
Vinolo, Mario A. R.
36472b53-5030-4ca6-bbb5-0918fb767011
Rodrigues, Hosana G.
6bfd2f76-eeb9-44af-b3da-ab19b8f0d1e1
Paschoal, Vivian A.
bcf93f87-e7c7-4f73-be3d-64493e988687
Torres, Rosângela P.
cd91fc72-b6e2-48f7-889f-bf312472d992
Mancini-Filho, Jorge
0b3bccee-44a5-4695-bfbb-5f6a317bcfbc
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Hatanaka, Elaine
a75419b0-65c9-496b-ba2e-62e716fd0965
Curi, Rui
87abee54-a75d-4e94-b9b9-2169d8f97fec

Magdalon, Juliana, Vinolo, Mario A. R., Rodrigues, Hosana G., Paschoal, Vivian A., Torres, Rosângela P., Mancini-Filho, Jorge, Calder, Philip C., Hatanaka, Elaine and Curi, Rui (2012) Oral administration of oleic or linoleic acids modulates the production of inflammatory mediators by rat macrophages. Lipids, 47 (8), 803-812. (doi:10.1007/s11745-012-3687-9). (PMID:22695743)

Record type: Article

Abstract

Oleic (OLA) and linoleic (LNA) acids are commonly consumed fatty acids and they can modulate the inflammatory response, in which macrophages play an important role. The aim of this study was to investigate the effects of these two fatty acids on the production of inflammatory mediators by macrophages. Rats received oral administration of water (control), OLA or LNA(0.22 g/kg body weight) daily for 10 days and peritoneal resident macrophages were then isolated. Subsequently, they were seeded in culture plates and the production of various inflammatory mediators was assessed. Oral administration with OLA decreased the production of IL-1b, IL-6 and CINC-2ab by resident macrophages and LNA decreased the production of IL-1b, IL-6 and VEGF in the absence of lipopolysaccharide (LPS), although it accelerated IL-1b release and decreased IL-10 synthesis when cells were stimulated with LPS. Neither fatty acid affected the production of superoxide anion, hydrogen peroxide, nitrite, TNF-a, PGE2, LTB4 or 15(S)-HETE.
Thus, OLA and LNA influence the production of several
inflammatory mediators by macrophages.

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More information

Published date: August 2012
Keywords: Fatty acids, inflammation, ROS, RNS, cytokines, VEGF, Eicosanoids
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 341634
URI: http://eprints.soton.ac.uk/id/eprint/341634
ISSN: 0024-4201
PURE UUID: ec269277-77ab-4cdb-8535-14e646ffd287
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 31 Jul 2012 11:30
Last modified: 15 Mar 2024 02:50

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Contributors

Author: Juliana Magdalon
Author: Mario A. R. Vinolo
Author: Hosana G. Rodrigues
Author: Vivian A. Paschoal
Author: Rosângela P. Torres
Author: Jorge Mancini-Filho
Author: Elaine Hatanaka
Author: Rui Curi

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