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Search for potential markers for prostate cancer diagnosis, prognosis and treatment in clinical tissue specimens using amine-specific isobaric tagging (iTRAQ) with two-dimensional liquid chromatography and tandem mass spectrometry

Search for potential markers for prostate cancer diagnosis, prognosis and treatment in clinical tissue specimens using amine-specific isobaric tagging (iTRAQ) with two-dimensional liquid chromatography and tandem mass spectrometry
Search for potential markers for prostate cancer diagnosis, prognosis and treatment in clinical tissue specimens using amine-specific isobaric tagging (iTRAQ) with two-dimensional liquid chromatography and tandem mass spectrometry
This study aimed to identify candidate new diagnosis and prognosis markers and medicinal targets of prostate cancer (PCa), using state of the art proteomics. A total of 20 prostate tissue specimens from 10 patients with benign prostatic hyperplasia (BPH) and 10 with PCa (Tumour Node Metastasis [TNM] stage T1?T3) were analyzed by isobaric stable isotope labeling (iTRAQ) and two-dimensional liquid chromatography?tandem mass spectrometry (2DLC?MS/MS) approaches using a hybrid quadrupole time-of-flight system (QqTOF). The study resulted in the reproducible identification of 825 nonredundant gene products (p ? 0.05) of which 30 exhibited up-regulation (?2-fold) and another 35 exhibited down-regulation (?0.5-fold) between the BPH and PCa specimens constituting a major contribution toward their global proteomic assessment. Selected findings were confirmed by immunohistochemical analysis of prostate tissue specimens. The proteins determined support existing knowledge and uncover novel and promising PCa biomarkers. The PCa proteome found can serve as a useful aid for the identification of improved diagnostic and prognostic markers and ultimately novel chemopreventive and therapeutic targets.
prostate cancer, proteomics, biomarkers, itraq, lc?ms
1535-3893
3146-3158
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Tyritzis, Stavros I.
50aad5bd-651e-4c2b-8d74-bd1601b696ae
Roumeliotis, Theodoros
f1284c98-b5eb-483e-9416-594c678e62fd
Zerefos, Panagiotis
f93881db-14e6-490f-b4b9-54d657b5cd29
Giannopoulou, Eugenia G.
db208413-12b1-42dc-b545-9b5c0a66c50d
Vlahou, Antonia
16e6be89-40a2-449a-abd2-6df6e4e0fbd8
Kossida, Sophia
63a28f92-3755-425b-a5b3-22a66264953e
Diaz, Jose
6559c867-d96f-41f9-ab4f-68969a5dec6d
Vourekas, Stavros
206a9e91-c91b-4f53-a490-34172a1b1daa
Tamvakopoulos, Constantin
8ade63d2-9331-4f09-ab1d-484af1023e03
Pavlakis, Kitty
cb774e11-a435-4584-9d43-2cd9690bc606
Sanoudou, Despina
eea6e657-1543-4588-8cf1-703cd5507fbe
Constantinides, Constantinos A.
83439178-53d1-4a30-8b42-5ecc198287f6
Garbis, Spiros D.
7067fd19-50c9-4d42-9611-f370289470bd
Tyritzis, Stavros I.
50aad5bd-651e-4c2b-8d74-bd1601b696ae
Roumeliotis, Theodoros
f1284c98-b5eb-483e-9416-594c678e62fd
Zerefos, Panagiotis
f93881db-14e6-490f-b4b9-54d657b5cd29
Giannopoulou, Eugenia G.
db208413-12b1-42dc-b545-9b5c0a66c50d
Vlahou, Antonia
16e6be89-40a2-449a-abd2-6df6e4e0fbd8
Kossida, Sophia
63a28f92-3755-425b-a5b3-22a66264953e
Diaz, Jose
6559c867-d96f-41f9-ab4f-68969a5dec6d
Vourekas, Stavros
206a9e91-c91b-4f53-a490-34172a1b1daa
Tamvakopoulos, Constantin
8ade63d2-9331-4f09-ab1d-484af1023e03
Pavlakis, Kitty
cb774e11-a435-4584-9d43-2cd9690bc606
Sanoudou, Despina
eea6e657-1543-4588-8cf1-703cd5507fbe
Constantinides, Constantinos A.
83439178-53d1-4a30-8b42-5ecc198287f6

Garbis, Spiros D., Tyritzis, Stavros I., Roumeliotis, Theodoros, Zerefos, Panagiotis, Giannopoulou, Eugenia G., Vlahou, Antonia, Kossida, Sophia, Diaz, Jose, Vourekas, Stavros, Tamvakopoulos, Constantin, Pavlakis, Kitty, Sanoudou, Despina and Constantinides, Constantinos A. (2008) Search for potential markers for prostate cancer diagnosis, prognosis and treatment in clinical tissue specimens using amine-specific isobaric tagging (iTRAQ) with two-dimensional liquid chromatography and tandem mass spectrometry. Journal of Proteome Research, 7 (8), 3146-3158. (doi:10.1021/pr800060r). (PMID:18553995)

Record type: Article

Abstract

This study aimed to identify candidate new diagnosis and prognosis markers and medicinal targets of prostate cancer (PCa), using state of the art proteomics. A total of 20 prostate tissue specimens from 10 patients with benign prostatic hyperplasia (BPH) and 10 with PCa (Tumour Node Metastasis [TNM] stage T1?T3) were analyzed by isobaric stable isotope labeling (iTRAQ) and two-dimensional liquid chromatography?tandem mass spectrometry (2DLC?MS/MS) approaches using a hybrid quadrupole time-of-flight system (QqTOF). The study resulted in the reproducible identification of 825 nonredundant gene products (p ? 0.05) of which 30 exhibited up-regulation (?2-fold) and another 35 exhibited down-regulation (?0.5-fold) between the BPH and PCa specimens constituting a major contribution toward their global proteomic assessment. Selected findings were confirmed by immunohistochemical analysis of prostate tissue specimens. The proteins determined support existing knowledge and uncover novel and promising PCa biomarkers. The PCa proteome found can serve as a useful aid for the identification of improved diagnostic and prognostic markers and ultimately novel chemopreventive and therapeutic targets.

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Published date: 18 June 2008
Keywords: prostate cancer, proteomics, biomarkers, itraq, lc?ms
Organisations: Cancer Sciences

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Local EPrints ID: 341682
URI: https://eprints.soton.ac.uk/id/eprint/341682
ISSN: 1535-3893
PURE UUID: 6ecf5c01-68da-44e6-bff2-c2a90754b229

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Date deposited: 02 Aug 2012 15:20
Last modified: 18 Jul 2017 05:33

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Contributors

Author: Stavros I. Tyritzis
Author: Theodoros Roumeliotis
Author: Panagiotis Zerefos
Author: Eugenia G. Giannopoulou
Author: Antonia Vlahou
Author: Sophia Kossida
Author: Jose Diaz
Author: Stavros Vourekas
Author: Constantin Tamvakopoulos
Author: Kitty Pavlakis
Author: Despina Sanoudou
Author: Constantinos A. Constantinides

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