Response of the symbiotic cnidarian Anthopleura elegantissima transcriptome to temperature and UV increase
Response of the symbiotic cnidarian Anthopleura elegantissima transcriptome to temperature and UV increase
Elevated temperature and solar radiation, including ultraviolet radiation, are now recognized as the primary environmental stresses that lead to mass cnidarian bleaching. This study takes a functional genomics approach to identifying genes that change expression soon after exposure to these stressors in the temperate sea anemone Anthopleura elegantissima that harbors Symbiodinium, the same genus of symbionts found in reef-building corals. Symbiotic anemones were subjected to elevated temperature or UV over a 24 h period. cDNA from these animals was hybridized to a 10,000-feature cDNA microarray of A. elegantissima. Overall 2.7% of the 10,000 features were found to be differentially expressed as a function of temperature or UV stress. Of the 86 features sequenced, 45% displayed significant homology to sequences in GenBank. There are 27 features that were differentially expressed in both stress conditions. Gene ontology analysis placed the differentially expressed genes in a wide range of categories including cytoskeleton organization and biogenesis, protein biosynthesis, cell proliferation, apoptosis and transport. This suggests that the early stress response to elevated temperature and UV involves essentially all aspects of host cellular regulation and machinery and that downstream cnidarian bleaching is a complex cellular response in host tissues.
Cnidarian bleaching, Functional genomics, Microarray, ea anemone, Symbiodinium, Symbiosis
283-289
Richier, Sophie
0fc0b375-6918-4c06-9d6f-229f6c4046ca
Rodriguez-Lanetty, Mauricio
ba3f75de-7949-42ef-adda-3b4d71a1d514
Schnitzler, Christine E.
f5a1c906-0be3-4f15-9354-5efcb5b5904e
Weis, Virginia M.
423fb770-9820-4f15-847a-5633f19064af
December 2008
Richier, Sophie
0fc0b375-6918-4c06-9d6f-229f6c4046ca
Rodriguez-Lanetty, Mauricio
ba3f75de-7949-42ef-adda-3b4d71a1d514
Schnitzler, Christine E.
f5a1c906-0be3-4f15-9354-5efcb5b5904e
Weis, Virginia M.
423fb770-9820-4f15-847a-5633f19064af
Richier, Sophie, Rodriguez-Lanetty, Mauricio, Schnitzler, Christine E. and Weis, Virginia M.
(2008)
Response of the symbiotic cnidarian Anthopleura elegantissima transcriptome to temperature and UV increase.
Comparative Biochemistry and Physiology Part D Genomics and Proteomics, 3 (4), .
(doi:10.1016/j.cbd.2008.08.001).
(PMID:20494848)
Abstract
Elevated temperature and solar radiation, including ultraviolet radiation, are now recognized as the primary environmental stresses that lead to mass cnidarian bleaching. This study takes a functional genomics approach to identifying genes that change expression soon after exposure to these stressors in the temperate sea anemone Anthopleura elegantissima that harbors Symbiodinium, the same genus of symbionts found in reef-building corals. Symbiotic anemones were subjected to elevated temperature or UV over a 24 h period. cDNA from these animals was hybridized to a 10,000-feature cDNA microarray of A. elegantissima. Overall 2.7% of the 10,000 features were found to be differentially expressed as a function of temperature or UV stress. Of the 86 features sequenced, 45% displayed significant homology to sequences in GenBank. There are 27 features that were differentially expressed in both stress conditions. Gene ontology analysis placed the differentially expressed genes in a wide range of categories including cytoskeleton organization and biogenesis, protein biosynthesis, cell proliferation, apoptosis and transport. This suggests that the early stress response to elevated temperature and UV involves essentially all aspects of host cellular regulation and machinery and that downstream cnidarian bleaching is a complex cellular response in host tissues.
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Published date: December 2008
Keywords:
Cnidarian bleaching, Functional genomics, Microarray, ea anemone, Symbiodinium, Symbiosis
Organisations:
Ocean Biochemistry & Ecosystems
Identifiers
Local EPrints ID: 341902
URI: http://eprints.soton.ac.uk/id/eprint/341902
ISSN: 1744-117X
PURE UUID: 64363f2d-7805-4b4a-b7b6-3676a6041031
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Date deposited: 10 Aug 2012 12:57
Last modified: 14 Mar 2024 11:45
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Contributors
Author:
Sophie Richier
Author:
Mauricio Rodriguez-Lanetty
Author:
Christine E. Schnitzler
Author:
Virginia M. Weis
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