The University of Southampton
University of Southampton Institutional Repository

Characterization of superoxide dismutases in anoxia- and hyperoxia-tolerant symbiotic cnidarians

Richier, Sophie, Merle, Pierre-Laurent, Furla, Paola, Pigozzi, Delphine, Sola, François and Allemand, Denis (2003) Characterization of superoxide dismutases in anoxia- and hyperoxia-tolerant symbiotic cnidarians Biochimica et Biophysica Acta (BBA) - Biomembranes, 1621, (1), pp. 84-91. (doi:10.1016/S0304-4165(03)00049-7). (PMID:12667614).

Record type: Article

Abstract

Many cnidarians, such as sea anemones, contain photosynthetic symbiotic dinoflagellates called zooxanthellae. During a light/dark cycle, the intratentacular O(2) state changes in minutes from hypoxia to hyperoxia (3-fold normoxia). To understand the origin of the high tolerance to these unusual oxic conditions, we have characterized superoxide dismutases (SODs) from the three cellular compartments (ectoderm, endoderm and zooxanthellae) of the Mediterranean sea anemone Anemonia viridis. The lowest SOD activity was found in ectodermal cells while endodermal cells and zooxanthellae showed a higher SOD activity. Two, seven and six SOD activity bands were identified on native PAGE in ectoderm, endoderm and zooxanthellae, respectively. A CuZnSOD was identified in both ectodermal and endodermal tissues. MnSODs were detected in all compartments with two different subcellular localizations. One band displays a classical mitochondrial localization, the three others being extramitochondrial. FeSODs present in zooxanthellae also appeared in endodermal host tissue. The isoelectric points of all SODs were distributed between 4 and 5. For comparative study, a similar analysis was performed on the whole homogenate of a scleractinian coral Stylophora pistillata. These results are discussed in the context of tolerance to hyperoxia and to the transition from anoxia to hyperoxia.

Full text not available from this repository.

More information

Published date: 7 April 2003
Organisations: Molecular and Cellular

Identifiers

Local EPrints ID: 341909
URI: http://eprints.soton.ac.uk/id/eprint/341909
ISSN: 0304-4165
PURE UUID: a9b95d84-1861-458f-978f-26101b27ea57

Catalogue record

Date deposited: 15 Aug 2012 13:44
Last modified: 18 Jul 2017 05:31

Export record

Altmetrics

Contributors

Author: Sophie Richier
Author: Pierre-Laurent Merle
Author: Paola Furla
Author: Delphine Pigozzi
Author: François Sola
Author: Denis Allemand

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×