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Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning

Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning
Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning
Doped diamond-like carbon (DLC) coatings offer potential antifouling surfaces against microbial and protein attachment. In particular, stainless steel surgical instruments are subject to tissue protein and resilient prion protein attachment, making decontamination methods used in sterile service departments ineffective, potentially increasing the risk of iatrogenic Creutzfeldt-Jakob disease during surgical procedures. This study examined the adsorption of proteins and prion-associated amyloid to doped DLC surfaces and the efficacy of commercial cleaning chemistries applied to these spiked surfaces, compared to titanium nitride coating and stainless steel. Surfaces inoculated with ME7-infected brain homogenate were visualised using SYPRO Ruby/Thioflavin T staining and modified epi-fluorescence microscopy before and after cleaning. Reduced protein and prion amyloid contamination was observed on the modified surfaces and subsequent decontamination efficacy improved. This highlights the potential for a new generation of coatings for surgical instruments to reduce the risk of iatrogenic CJD infection.

0892-7014
563-569
Secker, T.J.
16b0a878-984f-4272-bfaa-667c7c63023a
Hervé, R.
9baddc65-93cf-4a18-9388-088d60572b06
Zhao, Q.
61c6253e-34e6-4d29-8f4c-9e8c16dce0c2
Borisenko, K.B.
9e7cc1e7-983a-49cd-95f2-99654bfca5c0
Abel, E.W.
e693c0e2-8684-4ca7-9921-bfd8a2a6e63d
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Secker, T.J.
16b0a878-984f-4272-bfaa-667c7c63023a
Hervé, R.
9baddc65-93cf-4a18-9388-088d60572b06
Zhao, Q.
61c6253e-34e6-4d29-8f4c-9e8c16dce0c2
Borisenko, K.B.
9e7cc1e7-983a-49cd-95f2-99654bfca5c0
Abel, E.W.
e693c0e2-8684-4ca7-9921-bfd8a2a6e63d
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Secker, T.J., Hervé, R., Zhao, Q., Borisenko, K.B., Abel, E.W. and Keevil, C.W. (2012) Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning. Biofouling, 28 (6), 563-569. (doi:10.1080/08927014.2012.698387). (PMID:22694725)

Record type: Article

Abstract

Doped diamond-like carbon (DLC) coatings offer potential antifouling surfaces against microbial and protein attachment. In particular, stainless steel surgical instruments are subject to tissue protein and resilient prion protein attachment, making decontamination methods used in sterile service departments ineffective, potentially increasing the risk of iatrogenic Creutzfeldt-Jakob disease during surgical procedures. This study examined the adsorption of proteins and prion-associated amyloid to doped DLC surfaces and the efficacy of commercial cleaning chemistries applied to these spiked surfaces, compared to titanium nitride coating and stainless steel. Surfaces inoculated with ME7-infected brain homogenate were visualised using SYPRO Ruby/Thioflavin T staining and modified epi-fluorescence microscopy before and after cleaning. Reduced protein and prion amyloid contamination was observed on the modified surfaces and subsequent decontamination efficacy improved. This highlights the potential for a new generation of coatings for surgical instruments to reduce the risk of iatrogenic CJD infection.

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More information

Published date: June 2012
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 342189
URI: http://eprints.soton.ac.uk/id/eprint/342189
ISSN: 0892-7014
PURE UUID: edb42040-09be-4c2e-9c1d-aeffc72a6c77
ORCID for T.J. Secker: ORCID iD orcid.org/0000-0001-7168-8592
ORCID for R. Hervé: ORCID iD orcid.org/0000-0001-8838-6515
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 14 Aug 2012 16:00
Last modified: 16 Nov 2021 02:44

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Contributors

Author: T.J. Secker ORCID iD
Author: R. Hervé ORCID iD
Author: Q. Zhao
Author: K.B. Borisenko
Author: E.W. Abel
Author: C.W. Keevil ORCID iD

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