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Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication

Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication
Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication
Chlamydia trachomatis serovar L1/440/LN possesses a 7498bp plasmid which was designated pLGV440. The plasmid was cloned at the BamH1 site of pAT153 into Escherichia coli and the recombinant plasmid was designated pCTL1. A detailed restriction endonuclease map of pCTL1 was constructed. A fragment of the chlamydial plasmid was shown to function as a promoter in E. coli when placed upstream of the lacZ gene. The entire plasmid was sequenced by the chain termination method. Open reading frames were identified from the resulting consensus sequence together with a candidate for the plasmid origin of replication consisting of four perfect tandem repeats of a 22bp sequence, an A:T rich sequence and an open reading frame which could generate a 34.8kdal product. The predicted polypeptide products of the open reading frames were compared by computer with all reported protein sequences. Homology of the predicted polypeptide product of an open reading frame to the E. coli dnaB protein and the analogous product of gene 12 of bacteriophage P22 is described.
0305-1048
4053-4067
Hatt, Christopher
ec97b43c-e320-4451-aa38-74929ab62100
Ward, Michael E.
549d4ddc-4e70-4059-809d-dfe5d497348c
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Hatt, Christopher
ec97b43c-e320-4451-aa38-74929ab62100
Ward, Michael E.
549d4ddc-4e70-4059-809d-dfe5d497348c
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Hatt, Christopher, Ward, Michael E. and Clarke, Ian N. (1988) Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication. Nucleic Acids Research, 16 (9), 4053-4067. (PMID:6307876)

Record type: Article

Abstract

Chlamydia trachomatis serovar L1/440/LN possesses a 7498bp plasmid which was designated pLGV440. The plasmid was cloned at the BamH1 site of pAT153 into Escherichia coli and the recombinant plasmid was designated pCTL1. A detailed restriction endonuclease map of pCTL1 was constructed. A fragment of the chlamydial plasmid was shown to function as a promoter in E. coli when placed upstream of the lacZ gene. The entire plasmid was sequenced by the chain termination method. Open reading frames were identified from the resulting consensus sequence together with a candidate for the plasmid origin of replication consisting of four perfect tandem repeats of a 22bp sequence, an A:T rich sequence and an open reading frame which could generate a 34.8kdal product. The predicted polypeptide products of the open reading frames were compared by computer with all reported protein sequences. Homology of the predicted polypeptide product of an open reading frame to the E. coli dnaB protein and the analogous product of gene 12 of bacteriophage P22 is described.

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Published date: 11 May 1988
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 342329
URI: https://eprints.soton.ac.uk/id/eprint/342329
ISSN: 0305-1048
PURE UUID: 5713a2e0-6382-429f-a6c9-8afad6591b7f
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 06 Sep 2012 10:21
Last modified: 06 Jun 2018 13:19

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