The University of Southampton
University of Southampton Institutional Repository

Chemical modification of siRNA

Deleavey, Glen F., Watts, Jonathan K. and Damha, Masad J. (2009) Chemical modification of siRNA Current Protocols in Nucleic Acid Chemistry (doi:10.1002/0471142700.nc1603s39).

Record type: Article


The ability to manipulate the RNA interference (RNAi) machinery to specifically silence the expression of target genes could be a powerful therapeutic strategy. Since the discovery that RNAi can be triggered in mammalian cells by short double-stranded RNAs (small interfering RNA, siRNA), there has been a tremendous push by researchers, from academia to big pharma, to move siRNAs into clinical application. The challenges facing siRNA therapeutics are significant. The inherent properties of siRNAs (polyanionic, vulnerable to nuclease cleavage) make clinical application difficult due to poor cellular uptake and rapid clearance. Side effects of siRNAs have also proven to be a further complication. Fortunately, numerous chemical modification strategies have been identified that allow many of these obstacles to be overcome. This unit will present an overview of (1) the chemical modifications available to the nucleic acid chemist for modifying siRNAs, (2) the application of chemical modifications to address specific therapeutic obstacles, and (3) the factors that must be considered when assessing the activity of modified siRNAs

Full text not available from this repository.

More information

Published date: 2009
Organisations: Chemistry, Organic Chemistry: SCF


Local EPrints ID: 342432
ISSN: 1934-9270
PURE UUID: 27801ffa-4688-4e96-bfd4-462371083b06

Catalogue record

Date deposited: 29 Aug 2012 11:59
Last modified: 18 Jul 2017 05:28

Export record



Author: Glen F. Deleavey
Author: Jonathan K. Watts
Author: Masad J. Damha

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.