The University of Southampton
University of Southampton Institutional Repository

Simultaneous noncontact topography and electrochemical imaging by SECM/SICM featuring ion current feedback regulation

Simultaneous noncontact topography and electrochemical imaging by SECM/SICM featuring ion current feedback regulation
Simultaneous noncontact topography and electrochemical imaging by SECM/SICM featuring ion current feedback regulation
We described a hybrid system of scanning electrochemical microscopy (SECM) and scanning ion conductance microscopy (SICM) with ion current feedback nanopositioning control for simultaneous imaging of noncontact topography and spatial distribution of electrochemical species. A nanopipette/nanoring electrode probe provided submicrometer resolution of the electrochemical measurement on surfaces with complex topology. The SECM/SICM probe had an aperture radius of 220 nm. The inner and outer radii of the SECM Au nanoring electrode were 330 and 550 nm, respectively. Characterization of the probe was performed with scanning electron microscopy (SEM), cyclic voltammetry (CV), and approach curve measurements. SECM/SICM was applied to simultaneous imaging of topography and electrochemical responses of enzymes (horse radish peroxidase (HRP) and glucose oxidase (GOD)) and single live cells (A6 cells, superior cervical ganglion (SCG) cells, and cardiac myocytes). The measurements revealed the distribution of activity of the enzyme spots on uneven surfaces with submicrometer resolution. SECM/SICM acquired high resolution topographic images of cells together with the map of electrochemical signals. This combined technique was also applied to the evaluation of the permeation property of electroactive species through cellular membranes.
0002-7863
10118-10126
Takahashi, Yasufumi
5de59a5f-0c4a-4ae8-84b1-5a261fa49946
Shevchuk, Andrew I.
8388aa54-775a-47cf-b18b-a8ecf75ae996
Novak, Pavel
14838d74-6ee4-46b7-acf5-23bb58b2b68e
Murakami, Yumi
2c75cbed-9c6f-4475-94f6-5c30690d81ba
Shiku, Hitoshi
3a4e2dba-c977-4c03-9585-29636731b849
Korchev, Yuri E.
e53ab2e4-cef8-4439-9cef-b1ca04c3b489
Matsue, Tomokazu
cc56b1a2-ab4f-434d-af15-e1d60db0b13d
Takahashi, Yasufumi
5de59a5f-0c4a-4ae8-84b1-5a261fa49946
Shevchuk, Andrew I.
8388aa54-775a-47cf-b18b-a8ecf75ae996
Novak, Pavel
14838d74-6ee4-46b7-acf5-23bb58b2b68e
Murakami, Yumi
2c75cbed-9c6f-4475-94f6-5c30690d81ba
Shiku, Hitoshi
3a4e2dba-c977-4c03-9585-29636731b849
Korchev, Yuri E.
e53ab2e4-cef8-4439-9cef-b1ca04c3b489
Matsue, Tomokazu
cc56b1a2-ab4f-434d-af15-e1d60db0b13d

Takahashi, Yasufumi, Shevchuk, Andrew I., Novak, Pavel, Murakami, Yumi, Shiku, Hitoshi, Korchev, Yuri E. and Matsue, Tomokazu (2010) Simultaneous noncontact topography and electrochemical imaging by SECM/SICM featuring ion current feedback regulation. Journal of the American Chemical Society, 132 (29), 10118-10126. (doi:10.1021/ja1029478).

Record type: Article

Abstract

We described a hybrid system of scanning electrochemical microscopy (SECM) and scanning ion conductance microscopy (SICM) with ion current feedback nanopositioning control for simultaneous imaging of noncontact topography and spatial distribution of electrochemical species. A nanopipette/nanoring electrode probe provided submicrometer resolution of the electrochemical measurement on surfaces with complex topology. The SECM/SICM probe had an aperture radius of 220 nm. The inner and outer radii of the SECM Au nanoring electrode were 330 and 550 nm, respectively. Characterization of the probe was performed with scanning electron microscopy (SEM), cyclic voltammetry (CV), and approach curve measurements. SECM/SICM was applied to simultaneous imaging of topography and electrochemical responses of enzymes (horse radish peroxidase (HRP) and glucose oxidase (GOD)) and single live cells (A6 cells, superior cervical ganglion (SCG) cells, and cardiac myocytes). The measurements revealed the distribution of activity of the enzyme spots on uneven surfaces with submicrometer resolution. SECM/SICM acquired high resolution topographic images of cells together with the map of electrochemical signals. This combined technique was also applied to the evaluation of the permeation property of electroactive species through cellular membranes.

Full text not available from this repository.

More information

Published date: June 2010
Organisations: Optoelectronics Research Centre, Centre for Biological Sciences

Identifiers

Local EPrints ID: 342981
URI: http://eprints.soton.ac.uk/id/eprint/342981
ISSN: 0002-7863
PURE UUID: ff5c204b-1ca9-4f55-865f-9a4e95355b28

Catalogue record

Date deposited: 19 Sep 2012 10:29
Last modified: 07 Oct 2019 18:35

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×