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Characterisation of mouse limbal neurosphere cells: a potential cell source of functional neurons

Characterisation of mouse limbal neurosphere cells: a potential cell source of functional neurons
Characterisation of mouse limbal neurosphere cells: a potential cell source of functional neurons
BACKGROUND/AIMS:
To characterise the origin, ultrastructure and functional properties of corneal limbal neurospheres (LNS).
METHODS:
Limbal cells were isolated from the corneal limbus of adult mice and cultured in a serum-free sphere forming culture system. LNS were characterised by immunocytochemistry, Reverse-transcription-PCR and electron microscopy. LNS cells were also cocultured with neonatal mouse retinal cells. Phenotype and function were then assessed by immunofluorescence and a calcium influx/efflux assay.
RESULTS:
LNS cells displayed clonal growth and self-renewal, and expressed a wide range of stem cell and neural lineage markers. The acquisition of neural properties was concordant with expression of neural crest markers including CD34, Sca1, Sox9, Twist1, but not CD45. LNS exhibited similar morphology and microstructure to neurospheres derived from the central nervous system. Following culture in a conducive environment, the derived cells displayed mature neural markers and exhibited electrical excitability.
CONCLUSIONS:
Corneal limbal stromal progenitor cells are a potential and convenient autologous cell source to generate functional neurons.
0007-1161
1431-1437
Chen, Xiaoli
fba6c7fa-57f3-4f56-838e-fbb787004fec
Thomson, Heather
8914c4e4-b602-4ecc-a99d-897ed0fdd472
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Lotery, A.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
Chen, Xiaoli
fba6c7fa-57f3-4f56-838e-fbb787004fec
Thomson, Heather
8914c4e4-b602-4ecc-a99d-897ed0fdd472
Hossain, Parwez
563de5fc-84ad-4539-9228-bde0237eaf51
Lotery, A.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514

Chen, Xiaoli, Thomson, Heather, Hossain, Parwez and Lotery, A. (2012) Characterisation of mouse limbal neurosphere cells: a potential cell source of functional neurons. British Journal of Ophthalmology, 96 (11), 1431-1437. (doi:10.1136/bjophthalmol-2012-301546). (PMID:22952405)

Record type: Article

Abstract

BACKGROUND/AIMS:
To characterise the origin, ultrastructure and functional properties of corneal limbal neurospheres (LNS).
METHODS:
Limbal cells were isolated from the corneal limbus of adult mice and cultured in a serum-free sphere forming culture system. LNS were characterised by immunocytochemistry, Reverse-transcription-PCR and electron microscopy. LNS cells were also cocultured with neonatal mouse retinal cells. Phenotype and function were then assessed by immunofluorescence and a calcium influx/efflux assay.
RESULTS:
LNS cells displayed clonal growth and self-renewal, and expressed a wide range of stem cell and neural lineage markers. The acquisition of neural properties was concordant with expression of neural crest markers including CD34, Sca1, Sox9, Twist1, but not CD45. LNS exhibited similar morphology and microstructure to neurospheres derived from the central nervous system. Following culture in a conducive environment, the derived cells displayed mature neural markers and exhibited electrical excitability.
CONCLUSIONS:
Corneal limbal stromal progenitor cells are a potential and convenient autologous cell source to generate functional neurons.

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More information

Accepted/In Press date: 5 September 2012
Published date: November 2012
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 343200
URI: https://eprints.soton.ac.uk/id/eprint/343200
ISSN: 0007-1161
PURE UUID: ec23cab8-1b8e-42f4-8ba6-a1a421b512f6
ORCID for Parwez Hossain: ORCID iD orcid.org/0000-0002-3131-2395
ORCID for A. Lotery: ORCID iD orcid.org/0000-0001-5541-4305

Catalogue record

Date deposited: 27 Sep 2012 15:24
Last modified: 31 Jan 2019 01:36

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Contributors

Author: Xiaoli Chen
Author: Heather Thomson
Author: Parwez Hossain ORCID iD
Author: A. Lotery ORCID iD

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