The University of Southampton
University of Southampton Institutional Repository

Survival of Cryptosporidium parvum in faecal wastes and salad crops

Survival of Cryptosporidium parvum in faecal wastes and salad crops
Survival of Cryptosporidium parvum in faecal wastes and salad crops
Cryptosporidium parvum is a protozoan parasite of man and a range of animals. An essential stage of the life cycle is the formation in the gastrointestinal tract of oocysts, each containing four infectious sporozoites. Thick wall oocysts are shed in apparently normal or diarrhoetic faeces to contaminate soil and water, providing routes into the food chain. Desiccation and snap freezing are lethal to oocysts. Nevertheless, the robust coat affords significant protection against environmental stressors such as low pH or water activity, temperatures above freezing and a range of sanitising and oxidizing agents such as chlorine. C. parvum may therefore survive in the environment for extending periods of time, posing a threat to animals and man if ingested either directly in contaminated water or in uncooked/ improperly cooked foods which are cropped from contaminated land. This threat has only partially be characterized, due in part to the difficulty in recovering oocysts and other pathogens from complex matrices such as manures and salad leaf surfaces.

Accordingly, this study describes improved methods for the recovery of oocysts from faecal waste samples and salad crops, with recovery efficiencies of 40-80%. Using these improved tools, the results of oocyst survival studies in the stored wastes and on salad leaves are presented to show the suitability and drawbacks of current practices to minimize entry and spread of C. parvum in the food chain
1 84170 3273
15-24
Teagasc
Warnes, S.
f724f4bf-86cf-4b7b-bf0a-69ba86e0185c
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Duffy, G.
Warnes, S.
f724f4bf-86cf-4b7b-bf0a-69ba86e0185c
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Duffy, G.

Warnes, S. and Keevil, C.W. (2003) Survival of Cryptosporidium parvum in faecal wastes and salad crops. In, Duffy, G. (ed.) Cryptosporidium parvum in Food and Water. Dublin, IE. Teagasc, pp. 15-24.

Record type: Book Section

Abstract

Cryptosporidium parvum is a protozoan parasite of man and a range of animals. An essential stage of the life cycle is the formation in the gastrointestinal tract of oocysts, each containing four infectious sporozoites. Thick wall oocysts are shed in apparently normal or diarrhoetic faeces to contaminate soil and water, providing routes into the food chain. Desiccation and snap freezing are lethal to oocysts. Nevertheless, the robust coat affords significant protection against environmental stressors such as low pH or water activity, temperatures above freezing and a range of sanitising and oxidizing agents such as chlorine. C. parvum may therefore survive in the environment for extending periods of time, posing a threat to animals and man if ingested either directly in contaminated water or in uncooked/ improperly cooked foods which are cropped from contaminated land. This threat has only partially be characterized, due in part to the difficulty in recovering oocysts and other pathogens from complex matrices such as manures and salad leaf surfaces.

Accordingly, this study describes improved methods for the recovery of oocysts from faecal waste samples and salad crops, with recovery efficiencies of 40-80%. Using these improved tools, the results of oocyst survival studies in the stored wastes and on salad leaves are presented to show the suitability and drawbacks of current practices to minimize entry and spread of C. parvum in the food chain

Full text not available from this repository.

More information

Published date: 2003
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 344799
URI: http://eprints.soton.ac.uk/id/eprint/344799
ISBN: 1 84170 3273
PURE UUID: 9821e787-5fbd-487d-95d2-f9a0b5ce45a6
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 12 Dec 2012 15:28
Last modified: 18 Feb 2021 16:58

Export record

Contributors

Author: S. Warnes
Author: C.W. Keevil ORCID iD
Editor: G. Duffy

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×