The University of Southampton
University of Southampton Institutional Repository

Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish

Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish
Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish
Background: Estimation of the intake of oily fish at a population level is difficult. The measurement of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in biological samples may provide a useful biomarker of intake.
Objective: We identified the most appropriate biomarkers for the assessment of habitual oily fish intake and changes in intake by elucidating the dose- and time-dependent response of EPA and DHA incorporation into various biological samples that represent roles in fatty acid transport, function, and storage.
Design: This was a double-blind, randomized, controlled intervention trial in 204men andwomen that lasted 12 mo. EPA andDHA capsules were provided in a manner to reflect sporadic consumption of oily fish (ie, 1, 2, or 4 times/wk). EPA and DHAwere assessed at 9 time points over 12 mo in 9 sample types (red blood cells, mononuclear cells, platelets, buccal cells, adipose tissue, plasma phosphatidylcholine, triglycerides, cholesteryl esters, and nonesterified fatty acids).
Results: A dose response (P , 0.05) was observed for EPA and
DHA in all pools except for red blood cell EPA (P = 0.057). EPA and DHA measures in plasma phosphatidylcholine and platelets were best for the discrimination between different intakes (P , 0.0001). The rate of incorporation varied between sample types, with the time to maximal incorporation ranging from days (plasma phosphatidylcholine)to months (mononuclear cells) to .12 mo (adipose tissue).
Conclusions: Plasma phosphatidylcholine EPA plus DHAwas identified as the most suitable biomarker of acute changes in EPA and DHA intake, and platelet and mononuclear cell EPA plus DHA were the most suitable biomarkers of habitual intake. This trial was registered at Current Controlled Trials (www.controlled-trials.com) as ISRCTN48398526.
0002-9165
748-758
Browning, Lucy M.
43c173cf-96b5-4a07-8402-053f755967c5
Walker, Celia G.
6fcaeb25-36ce-4da3-976b-5d0f1fdddb86
Mander, Adrian P.
f5b31d18-3a5b-48c9-95dc-04e7364b8c0a
West, Annette L.
735dcd0d-c6f3-464c-99a2-fa691bd66900
Madden, Jackie
0771e352-d432-41ea-8a7e-4704c1efca46
Gambell, Joanna M.
f8e6c2f2-d128-4886-9b8e-3daeaf72032d
Young, Stephen
2428ea70-bf67-4975-b555-c8ba0d99058f
Wang, Laura
44eb49dd-47a7-47a5-b862-5bdba49f7448
Jebb, Susan A.
f90c6f0c-da8d-4e00-b40f-38d624fc2d38
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Browning, Lucy M.
43c173cf-96b5-4a07-8402-053f755967c5
Walker, Celia G.
6fcaeb25-36ce-4da3-976b-5d0f1fdddb86
Mander, Adrian P.
f5b31d18-3a5b-48c9-95dc-04e7364b8c0a
West, Annette L.
735dcd0d-c6f3-464c-99a2-fa691bd66900
Madden, Jackie
0771e352-d432-41ea-8a7e-4704c1efca46
Gambell, Joanna M.
f8e6c2f2-d128-4886-9b8e-3daeaf72032d
Young, Stephen
2428ea70-bf67-4975-b555-c8ba0d99058f
Wang, Laura
44eb49dd-47a7-47a5-b862-5bdba49f7448
Jebb, Susan A.
f90c6f0c-da8d-4e00-b40f-38d624fc2d38
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Browning, Lucy M., Walker, Celia G., Mander, Adrian P., West, Annette L., Madden, Jackie, Gambell, Joanna M., Young, Stephen, Wang, Laura, Jebb, Susan A. and Calder, Philip C. (2012) Incorporation of eicosapentaenoic and docosahexaenoic acids into lipid pools when given as supplements providing doses equivalent to typical intakes of oily fish. American Journal of Clinical Nutrition, 96 (4), 748-758. (doi:10.3945/ajcn.112.041343). (PMID:22932281)

Record type: Article

Abstract

Background: Estimation of the intake of oily fish at a population level is difficult. The measurement of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in biological samples may provide a useful biomarker of intake.
Objective: We identified the most appropriate biomarkers for the assessment of habitual oily fish intake and changes in intake by elucidating the dose- and time-dependent response of EPA and DHA incorporation into various biological samples that represent roles in fatty acid transport, function, and storage.
Design: This was a double-blind, randomized, controlled intervention trial in 204men andwomen that lasted 12 mo. EPA andDHA capsules were provided in a manner to reflect sporadic consumption of oily fish (ie, 1, 2, or 4 times/wk). EPA and DHAwere assessed at 9 time points over 12 mo in 9 sample types (red blood cells, mononuclear cells, platelets, buccal cells, adipose tissue, plasma phosphatidylcholine, triglycerides, cholesteryl esters, and nonesterified fatty acids).
Results: A dose response (P , 0.05) was observed for EPA and
DHA in all pools except for red blood cell EPA (P = 0.057). EPA and DHA measures in plasma phosphatidylcholine and platelets were best for the discrimination between different intakes (P , 0.0001). The rate of incorporation varied between sample types, with the time to maximal incorporation ranging from days (plasma phosphatidylcholine)to months (mononuclear cells) to .12 mo (adipose tissue).
Conclusions: Plasma phosphatidylcholine EPA plus DHAwas identified as the most suitable biomarker of acute changes in EPA and DHA intake, and platelet and mononuclear cell EPA plus DHA were the most suitable biomarkers of habitual intake. This trial was registered at Current Controlled Trials (www.controlled-trials.com) as ISRCTN48398526.

Full text not available from this repository.

More information

e-pub ahead of print date: 29 August 2012
Published date: October 2012
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 345245
URI: https://eprints.soton.ac.uk/id/eprint/345245
ISSN: 0002-9165
PURE UUID: c80762f2-4ba9-4d01-92f6-98f51296de08

Catalogue record

Date deposited: 14 Nov 2012 14:18
Last modified: 18 Jul 2017 05:11

Export record

Altmetrics

Contributors

Author: Lucy M. Browning
Author: Celia G. Walker
Author: Adrian P. Mander
Author: Annette L. West
Author: Jackie Madden
Author: Joanna M. Gambell
Author: Stephen Young
Author: Laura Wang
Author: Susan A. Jebb

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×