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Interaction between maternal and offspring diet to impair vascular function and oxidative balance in high fat fed male mice

Interaction between maternal and offspring diet to impair vascular function and oxidative balance in high fat fed male mice
Interaction between maternal and offspring diet to impair vascular function and oxidative balance in high fat fed male mice
Aims: to determine the impact of maternal and post-weaning consumption of a high fat diet on endothelium-dependent vasorelaxation and redox regulation in adult male mouse offspring.

Methods: female C57BL6J mice were fed an obesogenic high fat diet (HF, 45% kcal fat) or standard chow (C, 21% kcal fat) pre-conception and throughout pregnancy and lactation. Post-weaning, male offspring were continued on the same diet as their mothers or placed on the alternative diet to give 4 dietary groups (C/C, HF/C, C/HF and HF/HF) which were studied at 15 or 30 weeks of age.

Results: there were significant effects of maternal diet on offspring body weight (p<0.004), systolic blood pressure (p = 0.026) and endothelium-dependent relaxation to ACh (p = 0.004) and NO production (p = 0.005) measured in the femoral artery. With control for maternal diet there was also an effect of offspring post-weaning dietary fat to increase systolic blood pressure (p<0.0001) and reduce endothelium-dependent relaxation (p = 0.022) and ACh-mediated NO production (p = 0.007). There was also a significant impact of age (p<0.005). Redox balance was perturbed, with altered regulation of vascular enzymes involved in ROS/NO signalling.

Conclusions: maternal consumption of a HF diet is associated with changes in vascular function and oxidative balance in the offspring of similar magnitude to those seen with consumption of a high fat diet post-weaning. Further, this disadvantageous vascular phenotype is exacerbated by age to influence the risk of developing obesity, raised blood pressure and endothelial dysfunction in adult life
1932-6203
e0050671
Torrens, Christopher
15a35713-0651-4249-8227-5901e2cfcd22
Ethirajan, P.
03abb65f-fe37-481f-a003-d8de536bff9e
Bruce, K.D.
1ded890b-addf-45bd-ba59-dbaedaeee931
Cagampang, F.R.
45dedb84-dd74-47be-b480-bea0f8d0251d
Siow, R.C.
32bdc56b-669e-4bec-90dc-cd229dbd8654
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c
Mann, G.E.
1610a1ba-7f18-4a66-9966-69e5e7a3705d
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494
Torrens, Christopher
15a35713-0651-4249-8227-5901e2cfcd22
Ethirajan, P.
03abb65f-fe37-481f-a003-d8de536bff9e
Bruce, K.D.
1ded890b-addf-45bd-ba59-dbaedaeee931
Cagampang, F.R.
45dedb84-dd74-47be-b480-bea0f8d0251d
Siow, R.C.
32bdc56b-669e-4bec-90dc-cd229dbd8654
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Byrne, Christopher D.
1370b997-cead-4229-83a7-53301ed2a43c
Mann, G.E.
1610a1ba-7f18-4a66-9966-69e5e7a3705d
Clough, G.F.
9f19639e-a929-4976-ac35-259f9011c494

Torrens, Christopher, Ethirajan, P., Bruce, K.D., Cagampang, F.R., Siow, R.C., Hanson, Mark A., Byrne, Christopher D., Mann, G.E. and Clough, G.F. (2012) Interaction between maternal and offspring diet to impair vascular function and oxidative balance in high fat fed male mice. PLoS ONE, 7 (12), e0050671. (doi:10.1371/journal.pone.0050671). (PMID:23227196)

Record type: Article

Abstract

Aims: to determine the impact of maternal and post-weaning consumption of a high fat diet on endothelium-dependent vasorelaxation and redox regulation in adult male mouse offspring.

Methods: female C57BL6J mice were fed an obesogenic high fat diet (HF, 45% kcal fat) or standard chow (C, 21% kcal fat) pre-conception and throughout pregnancy and lactation. Post-weaning, male offspring were continued on the same diet as their mothers or placed on the alternative diet to give 4 dietary groups (C/C, HF/C, C/HF and HF/HF) which were studied at 15 or 30 weeks of age.

Results: there were significant effects of maternal diet on offspring body weight (p<0.004), systolic blood pressure (p = 0.026) and endothelium-dependent relaxation to ACh (p = 0.004) and NO production (p = 0.005) measured in the femoral artery. With control for maternal diet there was also an effect of offspring post-weaning dietary fat to increase systolic blood pressure (p<0.0001) and reduce endothelium-dependent relaxation (p = 0.022) and ACh-mediated NO production (p = 0.007). There was also a significant impact of age (p<0.005). Redox balance was perturbed, with altered regulation of vascular enzymes involved in ROS/NO signalling.

Conclusions: maternal consumption of a HF diet is associated with changes in vascular function and oxidative balance in the offspring of similar magnitude to those seen with consumption of a high fat diet post-weaning. Further, this disadvantageous vascular phenotype is exacerbated by age to influence the risk of developing obesity, raised blood pressure and endothelial dysfunction in adult life

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Published date: December 2012
Organisations: Human Development & Health

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Local EPrints ID: 345269
URI: http://eprints.soton.ac.uk/id/eprint/345269
ISSN: 1932-6203
PURE UUID: 8aff7777-c726-431a-8b46-230c9229adef
ORCID for Mark A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for Christopher D. Byrne: ORCID iD orcid.org/0000-0001-6322-7753
ORCID for G.F. Clough: ORCID iD orcid.org/0000-0002-6226-8964

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Date deposited: 15 Nov 2012 09:39
Last modified: 15 Mar 2024 03:07

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Contributors

Author: Christopher Torrens
Author: P. Ethirajan
Author: K.D. Bruce
Author: F.R. Cagampang
Author: R.C. Siow
Author: Mark A. Hanson ORCID iD
Author: G.E. Mann
Author: G.F. Clough ORCID iD

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