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Apolipoprotein E (apoE) uptake and distribution in mammalian cell lines is dependent upon source of apoE and can be monitored in living cells

Apolipoprotein E (apoE) uptake and distribution in mammalian cell lines is dependent upon source of apoE and can be monitored in living cells
Apolipoprotein E (apoE) uptake and distribution in mammalian cell lines is dependent upon source of apoE and can be monitored in living cells
As part of investigations of the cellular uptake of apolipoprotein E (apoE) relevant to Alzheimer's disease we have found that different preparations of apoE are handled differently by cells expressing the LDL-receptor. Comparing recombinant, cellular and native apoE, complexed with different preparations of lipid we find that only cellular and native apoE enter a vesicular compartment. Some, but not all of these apoE containing vesicles are lysosomes. In order to further examine the intracellular fate of apoE we demonstrate that apoE-Enhanced green fluorescent protein chimeric protein can be taken up from medium by recipient cells and tracked within these cells for extended periods.
apolipoprotein E, tau, ?vldl, enhanced green fluorescent protein, alzheimer's disease, uptake, apolipoprotein
0304-3940
69-73
Ljungberg, M.C.
e30f34cd-f5b1-403a-8a67-bbad2a015f0a
Asuni, A.A.
b1412b1b-9794-4705-aada-aed5d3da038f
Pearce, J.
2e64f1c2-a921-4c59-b43c-d89bf20e4d3e
Dayanandan, R.
7989ac93-7baf-45c2-9440-203bfca2cea0
März, W.
9cb63283-b6cc-4207-ad86-1e7fa4371b9a
Hoffmann, M.M.
b860f2b4-36df-438a-8c56-38c006005e89
Bertrand, P.
a8b0e28a-4f44-4c8f-80b5-afd1a3648d53
Siest, G.
43079935-7ad6-4f76-a4d4-3291aaba531f
Rupniak, H.T.R.
612ff7c4-13db-4a4a-98ec-db738ef011d9
Anderton, B.H.
7b040dc9-263d-4de6-820c-1ff7f5bba7c9
Huettinger, M.
9bced47d-b983-498c-aeac-21783f9f47f9
Lovestone, S.
482e0c1a-10cf-45fb-8631-bf32ca331104
Ljungberg, M.C.
e30f34cd-f5b1-403a-8a67-bbad2a015f0a
Asuni, A.A.
b1412b1b-9794-4705-aada-aed5d3da038f
Pearce, J.
2e64f1c2-a921-4c59-b43c-d89bf20e4d3e
Dayanandan, R.
7989ac93-7baf-45c2-9440-203bfca2cea0
März, W.
9cb63283-b6cc-4207-ad86-1e7fa4371b9a
Hoffmann, M.M.
b860f2b4-36df-438a-8c56-38c006005e89
Bertrand, P.
a8b0e28a-4f44-4c8f-80b5-afd1a3648d53
Siest, G.
43079935-7ad6-4f76-a4d4-3291aaba531f
Rupniak, H.T.R.
612ff7c4-13db-4a4a-98ec-db738ef011d9
Anderton, B.H.
7b040dc9-263d-4de6-820c-1ff7f5bba7c9
Huettinger, M.
9bced47d-b983-498c-aeac-21783f9f47f9
Lovestone, S.
482e0c1a-10cf-45fb-8631-bf32ca331104

Ljungberg, M.C., Asuni, A.A., Pearce, J., Dayanandan, R., März, W., Hoffmann, M.M., Bertrand, P., Siest, G., Rupniak, H.T.R., Anderton, B.H., Huettinger, M. and Lovestone, S. (2003) Apolipoprotein E (apoE) uptake and distribution in mammalian cell lines is dependent upon source of apoE and can be monitored in living cells. Neuroscience Letters, 341 (1), 69-73. (doi:10.1016/S0304-3940(03)00064-8).

Record type: Article

Abstract

As part of investigations of the cellular uptake of apolipoprotein E (apoE) relevant to Alzheimer's disease we have found that different preparations of apoE are handled differently by cells expressing the LDL-receptor. Comparing recombinant, cellular and native apoE, complexed with different preparations of lipid we find that only cellular and native apoE enter a vesicular compartment. Some, but not all of these apoE containing vesicles are lysosomes. In order to further examine the intracellular fate of apoE we demonstrate that apoE-Enhanced green fluorescent protein chimeric protein can be taken up from medium by recipient cells and tracked within these cells for extended periods.

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More information

Published date: April 2003
Keywords: apolipoprotein E, tau, ?vldl, enhanced green fluorescent protein, alzheimer's disease, uptake, apolipoprotein
Organisations: Centre for Biological Sciences

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Local EPrints ID: 345529
URI: http://eprints.soton.ac.uk/id/eprint/345529
ISSN: 0304-3940
PURE UUID: 2131dc3c-c9ce-422b-bf15-01c6d758d7e4

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Date deposited: 12 Feb 2013 16:13
Last modified: 16 Jul 2019 21:49

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Contributors

Author: M.C. Ljungberg
Author: A.A. Asuni
Author: J. Pearce
Author: R. Dayanandan
Author: W. März
Author: M.M. Hoffmann
Author: P. Bertrand
Author: G. Siest
Author: H.T.R. Rupniak
Author: B.H. Anderton
Author: M. Huettinger
Author: S. Lovestone

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