Expression of the MAST family of serine/threonine kinases
Expression of the MAST family of serine/threonine kinases
The Microtubule-Associated Serine/Threonine Kinase family (MAST1-4, and MAST-like) is characterised by the presence of a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain (PDZ). This latter domain gives the MAST family the capacity to scaffold its own kinase activity. In the present study we have profiled the mRNA for each member of the MAST family transcripts across various tissues, with particular focus on rodent brain. Reverse-transcriptase polymerase chain reaction (RT-PCR) has shown equivalent patterns of expression for MAST1 and 2 in multiple tissues. Both MAST3 and 4 show more distinct expression in several tissues, and MAST-like appears to be predominantly expressed in heart and testis. In situ hybridisation reveals overlapping expression of MAST1 and 2 in specific brain regions. In contrast, MAST3 shows selective expression in the striatum and cerebral cortex. MAST4 also exhibits distinct expression in oligodendrocytes of white matter containing brain regions. In keeping with previous results, this family member also shows increased expression in the hippocampus following seizure-like activity. Our analysis of MAST family expression provides support for the role of these kinases in a broad range of neural functions.
12-19
Garland, Patrick
1d24a0cc-81f2-4ef1-82bd-77d2510e59d6
Quraishe, Shmma
cfc3aed4-f120-41aa-9127-0fc26c657ad2
French, Pim
a7adbef4-4019-469f-8c9e-cfb3778cc53a
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca
21 February 2008
Garland, Patrick
1d24a0cc-81f2-4ef1-82bd-77d2510e59d6
Quraishe, Shmma
cfc3aed4-f120-41aa-9127-0fc26c657ad2
French, Pim
a7adbef4-4019-469f-8c9e-cfb3778cc53a
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca
Abstract
The Microtubule-Associated Serine/Threonine Kinase family (MAST1-4, and MAST-like) is characterised by the presence of a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain (PDZ). This latter domain gives the MAST family the capacity to scaffold its own kinase activity. In the present study we have profiled the mRNA for each member of the MAST family transcripts across various tissues, with particular focus on rodent brain. Reverse-transcriptase polymerase chain reaction (RT-PCR) has shown equivalent patterns of expression for MAST1 and 2 in multiple tissues. Both MAST3 and 4 show more distinct expression in several tissues, and MAST-like appears to be predominantly expressed in heart and testis. In situ hybridisation reveals overlapping expression of MAST1 and 2 in specific brain regions. In contrast, MAST3 shows selective expression in the striatum and cerebral cortex. MAST4 also exhibits distinct expression in oligodendrocytes of white matter containing brain regions. In keeping with previous results, this family member also shows increased expression in the hippocampus following seizure-like activity. Our analysis of MAST family expression provides support for the role of these kinases in a broad range of neural functions.
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Accepted/In Press date: 16 December 2007
e-pub ahead of print date: 23 December 2007
Published date: 21 February 2008
Organisations:
Centre for Biological Sciences
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Local EPrints ID: 345544
URI: http://eprints.soton.ac.uk/id/eprint/345544
ISSN: 0006-8993
PURE UUID: ecb55b85-3297-4568-ae29-d2b1a998f7d7
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Date deposited: 14 Mar 2016 13:33
Last modified: 15 Mar 2024 03:25
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Author:
Pim French
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