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Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies: sequence-specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE).

Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies: sequence-specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE).
Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies: sequence-specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE).
In the near future the number of SNPs identified and mapped will increase and the need for high throughput SNP typing will be paramount for comprehensive examination by association of the role of genomic regions in disease traits. A range of higher throughput methods for typing SNPs is now in routine use in many laboratories worldwide. In this report, we analyse the relative advantages and disadvantages of three such methods, TaqMan, PCR-SSOP, and ARMS-MADGE, currently in use in our laboratories. Throughputs achievable are similar, but there are major differences in cost and time for set-up, equipment, consumables, and staff time, which may determine the choice for individual laboratories.
allele typing, TaqMan, MADGE, SSOP
340-347
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Beghé, Bianca
950a3274-9729-4352-9309-edb772f7d278
Turner, Steve
5ece7027-a2ba-4cd7-a710-6f4b823dba9a
Hinks, Lesley J.
26543550-2e57-4947-9737-25c4c4ab1163
Day, Ian N. M.
4a300555-7eea-4f61-926e-3cdfb0fd78fe
Howell, W. Martin
ecbb0dd6-f904-4da2-a05d-e542862531f8
Holloway, John W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Beghé, Bianca
950a3274-9729-4352-9309-edb772f7d278
Turner, Steve
5ece7027-a2ba-4cd7-a710-6f4b823dba9a
Hinks, Lesley J.
26543550-2e57-4947-9737-25c4c4ab1163
Day, Ian N. M.
4a300555-7eea-4f61-926e-3cdfb0fd78fe
Howell, W. Martin
ecbb0dd6-f904-4da2-a05d-e542862531f8

Holloway, John W., Beghé, Bianca, Turner, Steve, Hinks, Lesley J., Day, Ian N. M. and Howell, W. Martin (1999) Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies: sequence-specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE). Human Mutation, 14 (4), 340-347. (doi:10.1002/(SICI)1098-1004(199910)14:4<340::AID-HUMU10>3.0.CO;2-Z). (PMID:10502782)

Record type: Article

Abstract

In the near future the number of SNPs identified and mapped will increase and the need for high throughput SNP typing will be paramount for comprehensive examination by association of the role of genomic regions in disease traits. A range of higher throughput methods for typing SNPs is now in routine use in many laboratories worldwide. In this report, we analyse the relative advantages and disadvantages of three such methods, TaqMan, PCR-SSOP, and ARMS-MADGE, currently in use in our laboratories. Throughputs achievable are similar, but there are major differences in cost and time for set-up, equipment, consumables, and staff time, which may determine the choice for individual laboratories.

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More information

e-pub ahead of print date: 28 September 1999
Published date: October 1999
Keywords: allele typing, TaqMan, MADGE, SSOP
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 345642
URI: http://eprints.soton.ac.uk/id/eprint/345642
PURE UUID: 767a0827-d7a0-42ad-a1a2-31a5e3824d08
ORCID for John W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 05 Dec 2012 11:48
Last modified: 07 Oct 2020 06:36

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