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Hydrodynamic shear stress to remove Listeria monocytogenesbiofilms from stainless steel and polytetrafluoroethylene surfaces

Hydrodynamic shear stress to remove Listeria monocytogenesbiofilms from stainless steel and polytetrafluoroethylene surfaces
Hydrodynamic shear stress to remove Listeria monocytogenesbiofilms from stainless steel and polytetrafluoroethylene surfaces
Aims: To calculate the shear stress needed to remove sessile Listeria monocytogenes cells from stainless steel (SS) and polytetrafluoroethylene (PTFE) surfaces.
Methods and Results: Listeria monocytogenes biofilms were formed on SS and PTFE surfaces. Shear stress was calculated using a radial flow chamber device and cells quantified by staining with 4?,6-diamidino-2-phenylindole. Results showed that shear stress between 24 and 144 N m2 removed up to 98% of cells from SS surfaces. PTFE presents a very hydrophobic surface, and a significant lower removal (P < 0?05) of only 63% was achieved; moreover, on PTFE discs, detachment of L. monocytogenes biofilms was more efficient at alower shear stress (between 8?6 and 34 N m2).
Conclusions: Water flow is more effective in removing L. monocytogenes biofilms from SS surfaces than from PTFE materials.
Significance and Impact of the Study: This work clearly demonstrates that water flow does not have the same efficiency in removing cells from differentmaterial surfaces and shows the need to optimize cleaning and sampling procedures by considering the conditions in which cells attach to surfaces and the physicochemistry of the surfaces.
1364-5072
256-265
Gião, M.S.
5638b770-3681-48b2-a9ae-9152b36ac504
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Gião, M.S.
5638b770-3681-48b2-a9ae-9152b36ac504
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Gião, M.S. and Keevil, C.W. (2012) Hydrodynamic shear stress to remove Listeria monocytogenesbiofilms from stainless steel and polytetrafluoroethylene surfaces. Journal of Applied Microbiology, 114 (1), 256-265. (doi:10.1111/jam.12032).

Record type: Article

Abstract

Aims: To calculate the shear stress needed to remove sessile Listeria monocytogenes cells from stainless steel (SS) and polytetrafluoroethylene (PTFE) surfaces.
Methods and Results: Listeria monocytogenes biofilms were formed on SS and PTFE surfaces. Shear stress was calculated using a radial flow chamber device and cells quantified by staining with 4?,6-diamidino-2-phenylindole. Results showed that shear stress between 24 and 144 N m2 removed up to 98% of cells from SS surfaces. PTFE presents a very hydrophobic surface, and a significant lower removal (P < 0?05) of only 63% was achieved; moreover, on PTFE discs, detachment of L. monocytogenes biofilms was more efficient at alower shear stress (between 8?6 and 34 N m2).
Conclusions: Water flow is more effective in removing L. monocytogenes biofilms from SS surfaces than from PTFE materials.
Significance and Impact of the Study: This work clearly demonstrates that water flow does not have the same efficiency in removing cells from differentmaterial surfaces and shows the need to optimize cleaning and sampling procedures by considering the conditions in which cells attach to surfaces and the physicochemistry of the surfaces.

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More information

Published date: 1 November 2012
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 345684
URI: http://eprints.soton.ac.uk/id/eprint/345684
ISSN: 1364-5072
PURE UUID: e40bdc28-20ff-43e9-b5a4-1903da3e3f95
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 28 Nov 2012 12:53
Last modified: 09 Nov 2021 03:05

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