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An analysis of polymer type and chain length for use as a biological composite graft extender in impaction bone grafting: a mechanical and biocompatibility study

Tayton, Edward, Fahmy, Sherif, Purcell, Matthew, Aarvold, Alexander, Smith, James O., Kalra, Spandan, Briscoe, Adam, Lanham, Stuart, Howdle, Steven, Shakesheff, Kevin, Dunlop, Douglas G. and Oreffo, Richard O.C. (2012) An analysis of polymer type and chain length for use as a biological composite graft extender in impaction bone grafting: a mechanical and biocompatibility study Journal of Biomedical Materials Research Part A, 100, (12), pp. 3211-3219. (doi:10.1002/jbm.a.34264). (PMID:22707404).

Record type: Article

Abstract

Impaction bone grafting (IBG) with human allograft remains the preferred approach for replacement of lost bone stock during revision hip surgery. Associated problems include cost, disease transmission, and stem subsidence. Synthetic grafts are therefore appealing, and ideally display similar mechanical characteristics as allograft, but with enhanced ability to form de novo bone. High and low molecular weight forms of three different polymers [poly(DL-lactide) (P(DL) LA), poly(DL-lactide-co-glycolide) (P(DL) LGA), and poly(?-caprolactone) (PCL)] were milled, impacted into discs, and then examined in a shear testing rig, in comparison to allograft. In addition, skeletal stem cells (SSCs) were combined with each of the milled polymers, followed by impaction and examination for cell viability and number, via fluorostaining and biochemical assays. The shear strengths of high/low mwt P(DL) LA, and high/low mwt P(DL) LGA were significantly higher than allograft (p < 0.01). High/low mwt PCL had significantly lower shear strengths (p < 0.01). WST-1 assay and fluorstaining indicated significantly increased cell viability on high mwt P(DL) LA and high mwt P(DL) LGA over allograft (p < 0.05). Mechanical and biochemical analysis indicated improved properties of high mwt P(DL) LA and high mwt P(DL) LGA over allograft. This study indicates the potential of these polymers for use as substitute human allograft, creating a living composition with SSC for application in IBG.

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More information

e-pub ahead of print date: 15 June 2012
Published date: December 2012
Keywords: stem cells, polymer, scaffolds, impaction bone grafting, tissue engineering
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 345791
URI: http://eprints.soton.ac.uk/id/eprint/345791
ISSN: 1549-3296
PURE UUID: 7e5e855b-780c-4ae7-a10c-03c5961e6cc4
ORCID for Stuart Lanham: ORCID iD orcid.org/0000-0002-4516-264X
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 03 Dec 2012 16:16
Last modified: 18 Jul 2017 05:07

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Contributors

Author: Edward Tayton
Author: Sherif Fahmy
Author: Matthew Purcell
Author: Alexander Aarvold
Author: James O. Smith
Author: Spandan Kalra
Author: Adam Briscoe
Author: Stuart Lanham ORCID iD
Author: Steven Howdle
Author: Kevin Shakesheff
Author: Douglas G. Dunlop

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