Protein expression of STRO-1 cells in response to different topographic features
Protein expression of STRO-1 cells in response to different topographic features
Human skeletal stem cells (STRO-1 positive) display distinct responses to different topographical features. On a flat surface, skeletal cells spread, and in vitro, they typically display a polarized, fibroblast-like morphology. However, on microgrooved surfaces, these cells prefer to stretch along the grooves forming a similar morphology to in vivo, bipolarized fibroblasts. In contrast, on nanopits, these cells display a polygonal and osteoblastic phenotype. We have examined mechanotransduction events of STRO-1 positive in response to fibroblastic, microgrooved and osteogenic, controlled disorder nanopit, topographies using proteomics after 3 days in culture. Protein expression profiles were analyzed by difference gel electrophoresis to identify proteins that showed modulation of expression in response to different topographic features to assess early decision events in these cells on these discrete topographies. After only 72 hours in culture, STRO-1 positive displayed differential regulations of families of proteins involved in cell migration and proliferation. The current study indicated that osteogenic decision specific events had already occurred. Runx2 was localized in nuclei of the skeletal stem cells on the osteogenic nanopits; however, few signaling pathway changes were observed. This study demonstrated that micro- and nanotopographies activated skeletal stem cells at different times and with distinct mechanotransduction profiles.
534603
Kantawong, Fahsai
d209298e-ac00-49f0-a9b9-39d1e82d5925
Robertson, Mary E.
b5a461db-f10d-4e6f-8fe5-26863a4a6aa0
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Burchmore, Richard J.
9db5ecb2-23e8-4a50-ad42-c41d3c414306
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
6 October 2011
Kantawong, Fahsai
d209298e-ac00-49f0-a9b9-39d1e82d5925
Robertson, Mary E.
b5a461db-f10d-4e6f-8fe5-26863a4a6aa0
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Burchmore, Richard J.
9db5ecb2-23e8-4a50-ad42-c41d3c414306
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Kantawong, Fahsai, Robertson, Mary E., Gadegaard, Nikolaj, Oreffo, Richard O.C., Burchmore, Richard J. and Dalby, Matthew J.
(2011)
Protein expression of STRO-1 cells in response to different topographic features.
Journal of Tissue Engineering, 2 (1), .
(doi:10.4061/2011/534603).
(PMID:7553908)
Abstract
Human skeletal stem cells (STRO-1 positive) display distinct responses to different topographical features. On a flat surface, skeletal cells spread, and in vitro, they typically display a polarized, fibroblast-like morphology. However, on microgrooved surfaces, these cells prefer to stretch along the grooves forming a similar morphology to in vivo, bipolarized fibroblasts. In contrast, on nanopits, these cells display a polygonal and osteoblastic phenotype. We have examined mechanotransduction events of STRO-1 positive in response to fibroblastic, microgrooved and osteogenic, controlled disorder nanopit, topographies using proteomics after 3 days in culture. Protein expression profiles were analyzed by difference gel electrophoresis to identify proteins that showed modulation of expression in response to different topographic features to assess early decision events in these cells on these discrete topographies. After only 72 hours in culture, STRO-1 positive displayed differential regulations of families of proteins involved in cell migration and proliferation. The current study indicated that osteogenic decision specific events had already occurred. Runx2 was localized in nuclei of the skeletal stem cells on the osteogenic nanopits; however, few signaling pathway changes were observed. This study demonstrated that micro- and nanotopographies activated skeletal stem cells at different times and with distinct mechanotransduction profiles.
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Published date: 6 October 2011
Organisations:
Human Development & Health
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Local EPrints ID: 345996
URI: http://eprints.soton.ac.uk/id/eprint/345996
ISSN: 2041-7314
PURE UUID: cfc1c344-fe0c-43eb-abc3-fffc3bf7f88c
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Date deposited: 11 Dec 2012 14:01
Last modified: 15 Mar 2024 03:04
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Author:
Fahsai Kantawong
Author:
Mary E. Robertson
Author:
Nikolaj Gadegaard
Author:
Richard J. Burchmore
Author:
Matthew J. Dalby
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