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Primer1: Primer design Web service for Tetra-Primer ARMS-PCR

Primer1: Primer design Web service for Tetra-Primer ARMS-PCR
Primer1: Primer design Web service for Tetra-Primer ARMS-PCR
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given sequence. The Primer1 software was developed originally for use in the context of restriction fragment length polymorphism analysis using gel electrophoresis. However, recent applications have been more diverse, reviewed here, and we present an overview of the Primer1 software for primer design and web-service. We have updated the Primer1 program, and provide more complete details of the implementation. We also provide test data and output. The program is now available on a new, efficient, LAMP web service for users at: http://primer1.soton.ac.uk/primer1.html
1875-0362
55-58
Collins, Andrew
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183
Collins, Andrew
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183

Collins, Andrew and Ke, Xiayi (2012) Primer1: Primer design Web service for Tetra-Primer ARMS-PCR. The Open Bioinformatics Journal, 6, 55-58. (doi:10.2174/1875036201206010055).

Record type: Article

Abstract

Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given sequence. The Primer1 software was developed originally for use in the context of restriction fragment length polymorphism analysis using gel electrophoresis. However, recent applications have been more diverse, reviewed here, and we present an overview of the Primer1 software for primer design and web-service. We have updated the Primer1 program, and provide more complete details of the implementation. We also provide test data and output. The program is now available on a new, efficient, LAMP web service for users at: http://primer1.soton.ac.uk/primer1.html

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More information

Published date: 30 November 2012
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 346184
URI: https://eprints.soton.ac.uk/id/eprint/346184
ISSN: 1875-0362
PURE UUID: 04ee008c-ed8d-4b1e-ab54-a596d25196d4
ORCID for Andrew Collins: ORCID iD orcid.org/0000-0001-7108-0771

Catalogue record

Date deposited: 14 Jan 2013 14:31
Last modified: 06 Jun 2018 13:11

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Contributors

Author: Andrew Collins ORCID iD
Author: Xiayi Ke

University divisions

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