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Functional characterization of bitter-taste receptors expressed in mammalian testis

Functional characterization of bitter-taste receptors expressed in mammalian testis
Functional characterization of bitter-taste receptors expressed in mammalian testis
Mammalian spermatogenesis and sperm maturation are susceptible to the effects of internal and external factors. However, how male germ cells interact with and respond to these elements including those potentially toxic substances is poorly understood. Here, we show that many bitter-taste receptors (T2rs), which are believed to function as gatekeepers in the oral cavity to detect and innately prevent the ingestion of poisonous bitter-tasting compounds, are expressed in mouse seminiferous tubules. Our in situ hybridization results indicate that Tas2r transcripts are expressed postmeiotically. Functional analysis showed that mouse spermatids and spermatozoa responded to both naturally occurring and synthetic bitter-tasting compounds by increasing intracellular free calcium concentrations, and individual male germ cells exhibited different ligand-activation profiles, indicating that each cell may express a unique subset of T2r receptors. These calcium responses could be suppressed by a specific bitter-tastant blocker or abolished by the knockout of the gene for the G protein subunit ?-gustducin. Taken together, our data strongly suggest that male germ cells, like taste bud cells in the oral cavity and solitary chemosensory cells in the airway, utilize T2r receptors to sense chemicals in the milieu that may affect sperm behavior and fertilization
1360-9947
17-28
Xu, J.
caa6a307-ce55-4e76-9da4-0d8be2895651
Cao, J.
f906efb8-4878-4419-867f-e787b289b00a
Iguchi, N.
b44f7f34-c413-4e1d-a768-86569ff7b0b7
Riethmacher, D.
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Huang, L.
32d24e6b-e6fd-4622-b257-b3f57855d1f1
Xu, J.
caa6a307-ce55-4e76-9da4-0d8be2895651
Cao, J.
f906efb8-4878-4419-867f-e787b289b00a
Iguchi, N.
b44f7f34-c413-4e1d-a768-86569ff7b0b7
Riethmacher, D.
1a0a0c2e-e94d-4d0a-a890-90107a2545bc
Huang, L.
32d24e6b-e6fd-4622-b257-b3f57855d1f1

Xu, J., Cao, J., Iguchi, N., Riethmacher, D. and Huang, L. (2013) Functional characterization of bitter-taste receptors expressed in mammalian testis. Molecular Human Reproduction, 19 (1), 17-28. (doi:10.1093/molehr/gas040). (PMID:22983952)

Record type: Article

Abstract

Mammalian spermatogenesis and sperm maturation are susceptible to the effects of internal and external factors. However, how male germ cells interact with and respond to these elements including those potentially toxic substances is poorly understood. Here, we show that many bitter-taste receptors (T2rs), which are believed to function as gatekeepers in the oral cavity to detect and innately prevent the ingestion of poisonous bitter-tasting compounds, are expressed in mouse seminiferous tubules. Our in situ hybridization results indicate that Tas2r transcripts are expressed postmeiotically. Functional analysis showed that mouse spermatids and spermatozoa responded to both naturally occurring and synthetic bitter-tasting compounds by increasing intracellular free calcium concentrations, and individual male germ cells exhibited different ligand-activation profiles, indicating that each cell may express a unique subset of T2r receptors. These calcium responses could be suppressed by a specific bitter-tastant blocker or abolished by the knockout of the gene for the G protein subunit ?-gustducin. Taken together, our data strongly suggest that male germ cells, like taste bud cells in the oral cavity and solitary chemosensory cells in the airway, utilize T2r receptors to sense chemicals in the milieu that may affect sperm behavior and fertilization

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Published date: January 2013
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 346377
URI: http://eprints.soton.ac.uk/id/eprint/346377
ISSN: 1360-9947
PURE UUID: 4be55434-8993-44f9-8cdd-c14d3c65a1a3
ORCID for D. Riethmacher: ORCID iD orcid.org/0000-0002-4206-5529

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Date deposited: 02 Jan 2013 09:22
Last modified: 05 Apr 2022 01:40

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Contributors

Author: J. Xu
Author: J. Cao
Author: N. Iguchi
Author: D. Riethmacher ORCID iD
Author: L. Huang

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