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Development of Zn-related necrosis in tobacco is enhanced by expressingAtHMA4and depends on the apoplastic Zn levels

Development of Zn-related necrosis in tobacco is enhanced by expressingAtHMA4and depends on the apoplastic Zn levels
Development of Zn-related necrosis in tobacco is enhanced by expressingAtHMA4and depends on the apoplastic Zn levels
AtHMA4 was previously shown to contribute to the control of Zn root-to-shoot translocation and tolerance to high Zn. However, heterologous expression of 35S::AtHMA4 in tobacco (Nicotiana tabacum cv. Xanthi) results in enhanced Zn sensitivity. This study provides a better understanding of the development of this Zn-sensitive phenotype and demonstrates that substantial modifications of Zn homeostasis occur due to AtHMA4 expression. We show that ectopically expressing AtHMA4 in tobacco results in overloading the root and leaf apoplast with Zn. The tissue and cellular distribution of Zn, monitored using Zinpyr-1, was altered in the AtHMA4-expressing plants compared with wild type. Increased loading of the leaf apoplast with Zn in AtHMA4 transformants induced necrosis; this appeared at lower levels of Zn supply in the transgenics compared with wild type. This study suggests that Zn concentration may be sensed in the apoplast of leaves, and if concentrations are above a certain threshold then particular groups of cells accumulate Zn and necrosis is initiated. Therefore, this could be considered as a mechanism for protecting the other parts of the photosynthetically active leaf from Zn toxicity.
biofortification, cell wall, P1B-ATPase, phytoremediation, zinc, zinpyr-1
0140-7791
Sieianowski, Oskar
6167c815-aa68-49d3-a44c-b85cef80d58b
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Weremczuk, Aleksandra
4c222dc7-886d-4349-a959-02ee375afb2d
Ruszcznska, Anna
e48fc3b4-e4ff-4f47-9090-225a69b6e86f
Bulska, Ewa
77015079-9ab5-46ef-a96b-58b6429b0387
Williams, Lorraine Elizabeth
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b
Sieianowski, Oskar
6167c815-aa68-49d3-a44c-b85cef80d58b
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Weremczuk, Aleksandra
4c222dc7-886d-4349-a959-02ee375afb2d
Ruszcznska, Anna
e48fc3b4-e4ff-4f47-9090-225a69b6e86f
Bulska, Ewa
77015079-9ab5-46ef-a96b-58b6429b0387
Williams, Lorraine Elizabeth
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b

Sieianowski, Oskar, Barabasz, Anna, Weremczuk, Aleksandra, Ruszcznska, Anna, Bulska, Ewa, Williams, Lorraine Elizabeth and Antosiewicz, Danuta Maria (2012) Development of Zn-related necrosis in tobacco is enhanced by expressingAtHMA4and depends on the apoplastic Zn levels. Plant, Cell and Environment. (doi:10.1111/pce.12041). (PMID:23170996)

Record type: Article

Abstract

AtHMA4 was previously shown to contribute to the control of Zn root-to-shoot translocation and tolerance to high Zn. However, heterologous expression of 35S::AtHMA4 in tobacco (Nicotiana tabacum cv. Xanthi) results in enhanced Zn sensitivity. This study provides a better understanding of the development of this Zn-sensitive phenotype and demonstrates that substantial modifications of Zn homeostasis occur due to AtHMA4 expression. We show that ectopically expressing AtHMA4 in tobacco results in overloading the root and leaf apoplast with Zn. The tissue and cellular distribution of Zn, monitored using Zinpyr-1, was altered in the AtHMA4-expressing plants compared with wild type. Increased loading of the leaf apoplast with Zn in AtHMA4 transformants induced necrosis; this appeared at lower levels of Zn supply in the transgenics compared with wild type. This study suggests that Zn concentration may be sensed in the apoplast of leaves, and if concentrations are above a certain threshold then particular groups of cells accumulate Zn and necrosis is initiated. Therefore, this could be considered as a mechanism for protecting the other parts of the photosynthetically active leaf from Zn toxicity.

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More information

e-pub ahead of print date: 13 December 2012
Keywords: biofortification, cell wall, P1B-ATPase, phytoremediation, zinc, zinpyr-1
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 346399
URI: http://eprints.soton.ac.uk/id/eprint/346399
ISSN: 0140-7791
PURE UUID: 700ffad7-dee3-45f5-8af7-1157a55da1a0

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Date deposited: 18 Dec 2012 16:50
Last modified: 14 Mar 2024 12:36

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Contributors

Author: Oskar Sieianowski
Author: Anna Barabasz
Author: Aleksandra Weremczuk
Author: Anna Ruszcznska
Author: Ewa Bulska
Author: Danuta Maria Antosiewicz

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