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Enhancing the osteogenic efficacy of human bone marrow aspirate: concentrating osteoprogenitors using wave-assisted filtration

Enhancing the osteogenic efficacy of human bone marrow aspirate: concentrating osteoprogenitors using wave-assisted filtration
Enhancing the osteogenic efficacy of human bone marrow aspirate: concentrating osteoprogenitors using wave-assisted filtration
Background: recent approaches have sought to harness the potential of stem cells to regenerate bone that is lost as a consequence of trauma or disease. Bone marrow aspirate (BMA) provides an autologous source of osteoprogenitors for such applications. However, previous studies indicated that the concentration of osteoprogenitors present in BMA is less than required for robust bone regeneration. We provide further evidence for the importance of BMA enrichment for skeletal tissue engineering strategies using a novel acoustic wave-facilitated filtration strategy to concentrate BMA for osteoprogenitors, clinically applicable for intraoperative orthopedic use.

Methods: femoral BMA from 15 patients of an elderly cohort was concentrated for the nucleated cell fraction against erythrocytes and excess plasma volume via size exclusion filtration facilitated by acoustic agitation. The effect of aspirate concentration was assessed by assays for colony formation, flow cytometry, multilineage differentiation and scaffold seeding efficiency.

Results: BMA was filtered to achieve a mean 4.2-fold reduction in volume with a corresponding enrichment of viable and functional osteoprogenitors, indicated by flow cytometry and assays for colony formation. Enhanced osteogenic and chondrogenic differentiation was observed using concentrated aspirate and enhanced cell-seeding efficiency onto allogeneic bone graft as an effect of osteoprogenitor concentration relative specifically to the concentration of erythrocytes in the aspirate.

Conclusions: these studies provide evidence for the importance of BMA nucleated cell concentration for both cell differentiation and cell seeding efficiency and demonstrate the potential of this approach for intraoperative application to enhance bone healing
bone marrow aspirate, bone marrow stem cells, bone regeneration, colony-forming unit-fibroblastoid, mesenchymal stem cells, osteoprogenitors, tissue engineering
1465-3249
242-252
Dawson, Jonathan I
b220fe76-498d-47be-9995-92da6c289cf3
Smith, James O.
027f2a5a-1966-4077-97a7-f70d2e6b06b2
Aarvold, Alexander
11dc317f-47fd-4b2c-b0a6-78688c679b5a
Ridgway, Jonathan N.
ad83317e-e695-409d-b8b1-d948f1a6bb48
Curran, Steven J.
67345a17-4c61-4455-86b0-3d3a774c9b65
Dunlop, Douglas G.
5f8d8b5c-e516-48b8-831f-c6e5529a52cc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dawson, Jonathan I
b220fe76-498d-47be-9995-92da6c289cf3
Smith, James O.
027f2a5a-1966-4077-97a7-f70d2e6b06b2
Aarvold, Alexander
11dc317f-47fd-4b2c-b0a6-78688c679b5a
Ridgway, Jonathan N.
ad83317e-e695-409d-b8b1-d948f1a6bb48
Curran, Steven J.
67345a17-4c61-4455-86b0-3d3a774c9b65
Dunlop, Douglas G.
5f8d8b5c-e516-48b8-831f-c6e5529a52cc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Dawson, Jonathan I, Smith, James O., Aarvold, Alexander, Ridgway, Jonathan N., Curran, Steven J., Dunlop, Douglas G. and Oreffo, Richard O.C. (2013) Enhancing the osteogenic efficacy of human bone marrow aspirate: concentrating osteoprogenitors using wave-assisted filtration. Cytotherapy, 15 (2), 242-252. (doi:10.1016/j.jcyt.2012.09.004). (PMID:23245952)

Record type: Article

Abstract

Background: recent approaches have sought to harness the potential of stem cells to regenerate bone that is lost as a consequence of trauma or disease. Bone marrow aspirate (BMA) provides an autologous source of osteoprogenitors for such applications. However, previous studies indicated that the concentration of osteoprogenitors present in BMA is less than required for robust bone regeneration. We provide further evidence for the importance of BMA enrichment for skeletal tissue engineering strategies using a novel acoustic wave-facilitated filtration strategy to concentrate BMA for osteoprogenitors, clinically applicable for intraoperative orthopedic use.

Methods: femoral BMA from 15 patients of an elderly cohort was concentrated for the nucleated cell fraction against erythrocytes and excess plasma volume via size exclusion filtration facilitated by acoustic agitation. The effect of aspirate concentration was assessed by assays for colony formation, flow cytometry, multilineage differentiation and scaffold seeding efficiency.

Results: BMA was filtered to achieve a mean 4.2-fold reduction in volume with a corresponding enrichment of viable and functional osteoprogenitors, indicated by flow cytometry and assays for colony formation. Enhanced osteogenic and chondrogenic differentiation was observed using concentrated aspirate and enhanced cell-seeding efficiency onto allogeneic bone graft as an effect of osteoprogenitor concentration relative specifically to the concentration of erythrocytes in the aspirate.

Conclusions: these studies provide evidence for the importance of BMA nucleated cell concentration for both cell differentiation and cell seeding efficiency and demonstrate the potential of this approach for intraoperative application to enhance bone healing

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e-pub ahead of print date: 12 December 2012
Published date: February 2013
Keywords: bone marrow aspirate, bone marrow stem cells, bone regeneration, colony-forming unit-fibroblastoid, mesenchymal stem cells, osteoprogenitors, tissue engineering
Organisations: Engineering Science Unit, Human Development & Health

Identifiers

Local EPrints ID: 346414
URI: http://eprints.soton.ac.uk/id/eprint/346414
ISSN: 1465-3249
PURE UUID: 353e631c-6b33-4e1c-8e56-de0c5ab92788
ORCID for Jonathan I Dawson: ORCID iD orcid.org/0000-0002-6712-0598
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

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Date deposited: 02 Jan 2013 10:32
Last modified: 01 Oct 2019 00:55

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Contributors

Author: James O. Smith
Author: Alexander Aarvold
Author: Jonathan N. Ridgway
Author: Steven J. Curran
Author: Douglas G. Dunlop

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