The University of Southampton
University of Southampton Institutional Repository

Epidemiology and clinical impact of Pseudomonas aeruginosa infection in cystic fibrosis using AP-PCR fingerprinting

Epidemiology and clinical impact of Pseudomonas aeruginosa infection in cystic fibrosis using AP-PCR fingerprinting
Epidemiology and clinical impact of Pseudomonas aeruginosa infection in cystic fibrosis using AP-PCR fingerprinting
Arbitrarily primed PCR (AP-PCR) was utilized to genetically fingerprint 252 Pseudomonas aeruginosa strains isolated from the sputa of 50 cystic fibrosis (CF) patients attending the Cork CF clinic over a period of 3 years. Ten distinct P. aeruginosa strains were identified and the distribution, temporal trends and clinical impact of colonization with these individual P. aeruginosa clones was studied. A number of random isolates from each AP-PCR group were analysed using pulsed field gel electrophoresis (PFGE) in order to confirm the discriminatory power of the AP-PCR technique. The majority of patients were colonized with a single strain over the time period of the study, but it was also possible to harbour two or more strains transiently or simultaneously. Four main strains were relatively evenly distributed throughout the CF population, and it was noted that patients from the same family or attending the same school tended to harbour the same P. aeruginosa clone. Disease severity was significantly associated with the age of the patient (P<0.001), clearly indicating an increase in severity with increase in age. The general clinical status of the CF patients was not significantly associated with the P. aeruginosa variant isolated from their sputa. Lung status was defined by FEV1 measurement and chest X-ray score (CXR). The non parametric Kruskal-Wallis significance test of FEV1, CXR and age by colonizing P. aeruginosa clone indicated that FEV1 (P=0.017), but not CXR (P=0.19) or age (P=0.842), differed significantly across the clones of P. aeruginosa isolated. Patients harbouring P. aeruginosa strains B, F or G clearly had lower FEV1 scores while those harbouring clones A, C, D or H generally had higher FEV1 scores. Thus, the sub-species variant of P. aeruginosa colonizing CF patients may be associated with the severity of progressive lung disease.
field gel-electrophoresis, polymerase chain-reaction, strains, colonization, diversity, sputum
0163-4453
151-158
Adams, C.
c8757610-ae90-4da0-81e2-158f760946a8
Morris-Quinn, M.
090d0c5f-bf90-415b-84e4-ba700931a10c
McConnell, F.
57ee2d0a-a98c-4cbc-aef8-9d4fb13cc425
West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968
Lucey, B.
6673ca0d-f2c1-4527-b4cb-876cdc8a04fd
Shortt, C.
729da927-8090-435d-9c00-7c48b911c09d
Cryan, B.
e0912478-7486-4009-9304-08e0980a4dc0
Watson, J.B.G.
69939291-8b6b-4d1d-b9e3-c6a363774b62
O'Gara, F.
f78c890a-1a3b-4436-bf7f-baa2e6df7f7b
Adams, C.
c8757610-ae90-4da0-81e2-158f760946a8
Morris-Quinn, M.
090d0c5f-bf90-415b-84e4-ba700931a10c
McConnell, F.
57ee2d0a-a98c-4cbc-aef8-9d4fb13cc425
West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968
Lucey, B.
6673ca0d-f2c1-4527-b4cb-876cdc8a04fd
Shortt, C.
729da927-8090-435d-9c00-7c48b911c09d
Cryan, B.
e0912478-7486-4009-9304-08e0980a4dc0
Watson, J.B.G.
69939291-8b6b-4d1d-b9e3-c6a363774b62
O'Gara, F.
f78c890a-1a3b-4436-bf7f-baa2e6df7f7b

Adams, C., Morris-Quinn, M., McConnell, F., West, J., Lucey, B., Shortt, C., Cryan, B., Watson, J.B.G. and O'Gara, F. (1998) Epidemiology and clinical impact of Pseudomonas aeruginosa infection in cystic fibrosis using AP-PCR fingerprinting. Journal of Infection, 37 (2), 151-158. (doi:10.1016/S0163-4453(98)80170-9). (PMID:9821090)

Record type: Article

Abstract

Arbitrarily primed PCR (AP-PCR) was utilized to genetically fingerprint 252 Pseudomonas aeruginosa strains isolated from the sputa of 50 cystic fibrosis (CF) patients attending the Cork CF clinic over a period of 3 years. Ten distinct P. aeruginosa strains were identified and the distribution, temporal trends and clinical impact of colonization with these individual P. aeruginosa clones was studied. A number of random isolates from each AP-PCR group were analysed using pulsed field gel electrophoresis (PFGE) in order to confirm the discriminatory power of the AP-PCR technique. The majority of patients were colonized with a single strain over the time period of the study, but it was also possible to harbour two or more strains transiently or simultaneously. Four main strains were relatively evenly distributed throughout the CF population, and it was noted that patients from the same family or attending the same school tended to harbour the same P. aeruginosa clone. Disease severity was significantly associated with the age of the patient (P<0.001), clearly indicating an increase in severity with increase in age. The general clinical status of the CF patients was not significantly associated with the P. aeruginosa variant isolated from their sputa. Lung status was defined by FEV1 measurement and chest X-ray score (CXR). The non parametric Kruskal-Wallis significance test of FEV1, CXR and age by colonizing P. aeruginosa clone indicated that FEV1 (P=0.017), but not CXR (P=0.19) or age (P=0.842), differed significantly across the clones of P. aeruginosa isolated. Patients harbouring P. aeruginosa strains B, F or G clearly had lower FEV1 scores while those harbouring clones A, C, D or H generally had higher FEV1 scores. Thus, the sub-species variant of P. aeruginosa colonizing CF patients may be associated with the severity of progressive lung disease.

Full text not available from this repository.

More information

Published date: 1998
Keywords: field gel-electrophoresis, polymerase chain-reaction, strains, colonization, diversity, sputum
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 346425
URI: https://eprints.soton.ac.uk/id/eprint/346425
ISSN: 0163-4453
PURE UUID: b5408109-4e76-4ee5-8a16-e7632b7b98c3

Catalogue record

Date deposited: 26 Feb 2013 13:05
Last modified: 18 Jul 2017 05:04

Export record

Altmetrics

Contributors

Author: C. Adams
Author: M. Morris-Quinn
Author: F. McConnell
Author: J. West
Author: B. Lucey
Author: C. Shortt
Author: B. Cryan
Author: J.B.G. Watson
Author: F. O'Gara

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×