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A DNA diagnostic biosensor: development, characterisation and performance

A DNA diagnostic biosensor: development, characterisation and performance
A DNA diagnostic biosensor: development, characterisation and performance
The aim of this work is to develop a sensor for specific DNA sequences, using non-complex synthetic single-stranded oligonucleotides as a model system. A capacitance-based sensor for the direct detection of DNA sequences is described. Hybridisation of analyte DNA with immobilised DNA on the silicon surface induces charge effects, altering the dielectric properties of the biolayer, and can be detected by the associated change in the measured capacitance. DNA has been immobilised on a silicon electrode either by passive adsorption or covalent coupling via 4-aminobutyldimethylmethoxysilane (4-ABDMMS). The work presented here introduces a colourimetric immunodetection technique for the evaluation of the immobilisation process and describes the electrical characterisation and performance of three silicon-based sequence-specific DNA sensors. These sensors consisted of a standard electrolyte-insulator-semiconductor (EIS) structure with covalently bound probe DNA, a mechanically degraded structure with passively adsorbed probe DNA and a mechanically degraded structure with covalently bound probe DNA. The last device had an improved signal to noise ratio and was, therefore, used to construct a standard curve, revealing a detection Limit of 100 pmol DNA. On addition of analyte DNA, then was a decrease in measured capacitance. This response was fast, specific and required no addition of mediators to enhance or amplify the signal. This device can be optimised for the detection of complex sequences.
dna, hybridisation, silicon, capacitance, sensor, capacitive biosensor, hybridization, immobilization, sensor recognition, electrode, surface, damage
0925-4005
100-108
Berney, H.
bea83ffc-cbf9-4211-8d7a-4f0f7c9f87c5
West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968
Haefele, E.
8fc83a47-6fd2-4662-81c6-6aebf51a112e
Alderman, J.
9b9d56b3-8d96-4f09-85f2-ac6343427c85
Lane, W.
0b9a4c80-493c-42a6-a207-777f9501943a
Collins, J.K.
5b1439ca-39c7-41cd-a99f-328d91bb4119
Berney, H.
bea83ffc-cbf9-4211-8d7a-4f0f7c9f87c5
West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968
Haefele, E.
8fc83a47-6fd2-4662-81c6-6aebf51a112e
Alderman, J.
9b9d56b3-8d96-4f09-85f2-ac6343427c85
Lane, W.
0b9a4c80-493c-42a6-a207-777f9501943a
Collins, J.K.
5b1439ca-39c7-41cd-a99f-328d91bb4119

Berney, H., West, J., Haefele, E., Alderman, J., Lane, W. and Collins, J.K. (2000) A DNA diagnostic biosensor: development, characterisation and performance. Sensors and Actuators B: Chemical, 68 (1-3), 100-108. (doi:10.1016/S0925-4005(00)00468-8).

Record type: Article

Abstract

The aim of this work is to develop a sensor for specific DNA sequences, using non-complex synthetic single-stranded oligonucleotides as a model system. A capacitance-based sensor for the direct detection of DNA sequences is described. Hybridisation of analyte DNA with immobilised DNA on the silicon surface induces charge effects, altering the dielectric properties of the biolayer, and can be detected by the associated change in the measured capacitance. DNA has been immobilised on a silicon electrode either by passive adsorption or covalent coupling via 4-aminobutyldimethylmethoxysilane (4-ABDMMS). The work presented here introduces a colourimetric immunodetection technique for the evaluation of the immobilisation process and describes the electrical characterisation and performance of three silicon-based sequence-specific DNA sensors. These sensors consisted of a standard electrolyte-insulator-semiconductor (EIS) structure with covalently bound probe DNA, a mechanically degraded structure with passively adsorbed probe DNA and a mechanically degraded structure with covalently bound probe DNA. The last device had an improved signal to noise ratio and was, therefore, used to construct a standard curve, revealing a detection Limit of 100 pmol DNA. On addition of analyte DNA, then was a decrease in measured capacitance. This response was fast, specific and required no addition of mediators to enhance or amplify the signal. This device can be optimised for the detection of complex sequences.

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More information

Published date: 25 August 2000
Additional Information: ISI Document Delivery No.: 352LQ Times Cited: 114 Cited Reference Count: 24 Berney, H West, J Haefele, E Alderman, J Lane, W Collins, JK Eurosensors XIII Meeting Sep 12-15, 1999 The hague, netherlands Elsevier science sa Lausanne
Keywords: dna, hybridisation, silicon, capacitance, sensor, capacitive biosensor, hybridization, immobilization, sensor recognition, electrode, surface, damage
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 346426
URI: https://eprints.soton.ac.uk/id/eprint/346426
ISSN: 0925-4005
PURE UUID: 5f3fd5f7-7009-4e58-bb71-f498cfae5ae9

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Date deposited: 26 Feb 2013 13:33
Last modified: 18 Jul 2017 05:04

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Contributors

Author: H. Berney
Author: J. West
Author: E. Haefele
Author: J. Alderman
Author: W. Lane
Author: J.K. Collins

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