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Silicon microstructure arrays for DNA extraction by solid phase sample contacting at high flow rates

Silicon microstructure arrays for DNA extraction by solid phase sample contacting at high flow rates
Silicon microstructure arrays for DNA extraction by solid phase sample contacting at high flow rates
Rapid and large sample volume processing capabilities are required for many clinical and environmental point of care genetic diagnostics scenarios. In this paper we describe the development of a silicon monolithic device for high flow rate DNA extraction. The silicon presents silica surfaces and with chaotropic salt solutions can be used for solid phase extraction. The microfluidic device was designed with the aid of simulation methods and consists of a chamber containing a densely packed alternating array of teardrop-shaped microstructures. The microstructures repeatedly separate and recombine flows, and produce a near homogeneous flow distribution to favour DNA contact with the large silica surface areas. Extremely high flow rate operation (e.g. 10 mL min-1 can be achieved without device failure. Low DNA loading and elution flow rates C At high flow rates the DNA extraction capability is retained and is independent of were used to determine a binding capacity of 57 ± 15 ng cm-2 the sample DNA concentration. High flow rate operation is preferred for the rapid extraction of rare DNA species from large sample volumes, and makes the device ideal for inclusion within a fully integrated genetic diagnostics system.
DNA, solid phase extraction, microfluidics, silicon microstructures, monolithic device, purification, amplification, microchip, chip, rna, thiocyanate enrichment sequences, systems, ecdm
0925-4005
664-671
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Boerlin, Martin
754ab514-5338-4b30-8ee8-4da4b414b512
Jadhav, Amol D.
6b6a5ce3-1de0-470f-8068-493903f26825
Clancy, Eoin
2a281a07-e3a1-43a2-961a-d8cd4df2878b
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Boerlin, Martin
754ab514-5338-4b30-8ee8-4da4b414b512
Jadhav, Amol D.
6b6a5ce3-1de0-470f-8068-493903f26825
Clancy, Eoin
2a281a07-e3a1-43a2-961a-d8cd4df2878b

West, Jonathan, Boerlin, Martin, Jadhav, Amol D. and Clancy, Eoin (2007) Silicon microstructure arrays for DNA extraction by solid phase sample contacting at high flow rates. Sensors and Actuators B: Chemical, 126 (2), 664-671. (doi:10.1016/j.snb.2007.04.022).

Record type: Article

Abstract

Rapid and large sample volume processing capabilities are required for many clinical and environmental point of care genetic diagnostics scenarios. In this paper we describe the development of a silicon monolithic device for high flow rate DNA extraction. The silicon presents silica surfaces and with chaotropic salt solutions can be used for solid phase extraction. The microfluidic device was designed with the aid of simulation methods and consists of a chamber containing a densely packed alternating array of teardrop-shaped microstructures. The microstructures repeatedly separate and recombine flows, and produce a near homogeneous flow distribution to favour DNA contact with the large silica surface areas. Extremely high flow rate operation (e.g. 10 mL min-1 can be achieved without device failure. Low DNA loading and elution flow rates C At high flow rates the DNA extraction capability is retained and is independent of were used to determine a binding capacity of 57 ± 15 ng cm-2 the sample DNA concentration. High flow rate operation is preferred for the rapid extraction of rare DNA species from large sample volumes, and makes the device ideal for inclusion within a fully integrated genetic diagnostics system.

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More information

Published date: 1 October 2007
Keywords: DNA, solid phase extraction, microfluidics, silicon microstructures, monolithic device, purification, amplification, microchip, chip, rna, thiocyanate enrichment sequences, systems, ecdm
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 346433
URI: http://eprints.soton.ac.uk/id/eprint/346433
ISSN: 0925-4005
PURE UUID: b9e9bd09-c46d-4abe-a478-095784490780
ORCID for Jonathan West: ORCID iD orcid.org/0000-0002-5709-6790

Catalogue record

Date deposited: 26 Feb 2013 14:01
Last modified: 15 Mar 2024 03:43

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Contributors

Author: Jonathan West ORCID iD
Author: Martin Boerlin
Author: Amol D. Jadhav
Author: Eoin Clancy

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