The University of Southampton
University of Southampton Institutional Repository

Toward a PCR-independent molecular diagnosis of veterinary and medically relevant pathogenic organisms

Toward a PCR-independent molecular diagnosis of veterinary and medically relevant pathogenic organisms
Toward a PCR-independent molecular diagnosis of veterinary and medically relevant pathogenic organisms
Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.
aspergillus, candida, reverse line blot, pcr, diagnostics
0077-8923
391-393
Partington, Matthew W.
e11e8b3e-0cd8-4348-8e8b-a6ee2f9c2ddd
Masic, Dino
a66e8ffd-5829-46ec-9170-8f02a55eae7d
Nicholson, Audrey
debd7fe3-17b4-40d4-abaa-202e764f0704
Perry, John
1ab15608-bec6-4a0a-86ad-a9beb1f59e14
Berrington, Andrew
1dbf2b33-c93e-4fe9-bd9b-5c4a90c58c17
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Sparagano, Oliver A.E.
33d85c40-0f2a-4202-9749-411ee4abc982
Partington, Matthew W.
e11e8b3e-0cd8-4348-8e8b-a6ee2f9c2ddd
Masic, Dino
a66e8ffd-5829-46ec-9170-8f02a55eae7d
Nicholson, Audrey
debd7fe3-17b4-40d4-abaa-202e764f0704
Perry, John
1ab15608-bec6-4a0a-86ad-a9beb1f59e14
Berrington, Andrew
1dbf2b33-c93e-4fe9-bd9b-5c4a90c58c17
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Sparagano, Oliver A.E.
33d85c40-0f2a-4202-9749-411ee4abc982

Partington, Matthew W., Masic, Dino, Nicholson, Audrey, Perry, John, Berrington, Andrew, West, Jonathan and Sparagano, Oliver A.E. (2008) Toward a PCR-independent molecular diagnosis of veterinary and medically relevant pathogenic organisms. [in special issue: Animal Biodiversity and Emerging Diseases Prediction and Prevention] Annals of the New York Academy of Sciences, 1149, 391-393. (doi:10.1196/annals.1428.010). (PMID:19120258)

Record type: Article

Abstract

Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.

Full text not available from this repository.

More information

e-pub ahead of print date: 15 December 2008
Published date: December 2008
Keywords: aspergillus, candida, reverse line blot, pcr, diagnostics
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 346438
URI: https://eprints.soton.ac.uk/id/eprint/346438
ISSN: 0077-8923
PURE UUID: 49071f86-cd48-496a-baa6-449847ad0831

Catalogue record

Date deposited: 11 Feb 2013 15:30
Last modified: 18 Jul 2017 05:04

Export record

Altmetrics

Contributors

Author: Matthew W. Partington
Author: Dino Masic
Author: Audrey Nicholson
Author: John Perry
Author: Andrew Berrington
Author: Jonathan West
Author: Oliver A.E. Sparagano

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×