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Lipid nanotubule fabrication by microfluidic tweezing

Lipid nanotubule fabrication by microfluidic tweezing
Lipid nanotubule fabrication by microfluidic tweezing
There is currently great interest in the development of lipid enclosed systems with complex geometrical arrangements that mimic cellular compartments. With biochemical functionalization, these soft matter devices can be used to probe deeper into life's transport dominated biochemical operations. In this paper, we present a novel tool for machining lipid nanotubules by microfluidic tweezing. A bilayer poly(dimethylsiloxane) (PDMS) device was designed with a lipid reservoir that was loaded by capillary action for lipid film deposition. The lipid reservoir is vertically separated from an upper flow for controlled material wetting and the formation of giant tubule bodies. Three fluidic paths are interfaced for introduction of the giant tubules into the high velocity center of a parabolic flow profile for exposure to hydrodynamic shear stresses. At local velocities approximating 2 mm s(-1), a 300-500 nm diameter jet of lipid material was tweezed from the giant tubule body and elongated with the flow. The high velocity flow provides uniform drag for the rapid and continuous fabrication of lipid nanotubules with tremendous axial ratios. Below a critical velocity, a remarkable shape transformation occurred and the projected lipid tubule grew until a constant 3.6 mu m diameter tubule was attained. These lipid tubules could be wired for the construction of advanced lifelike bioreactor systems.
vesicle networks, electrophoretic transport, phospholipid membranes, surfactant liposomes, bilayers, tubules, device containers, extrusion
0743-7463
6754-6758
West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968
Manz, A.
d8d789e5-7066-4583-81f2-9d0410d3bbdc
Dittrich, P. S.
1ae53031-0f98-47ec-9dd6-e6387ac6d88b
West, J.
f1c2e060-16c3-44c0-af70-242a1c58b968
Manz, A.
d8d789e5-7066-4583-81f2-9d0410d3bbdc
Dittrich, P. S.
1ae53031-0f98-47ec-9dd6-e6387ac6d88b

West, J., Manz, A. and Dittrich, P. S. (2008) Lipid nanotubule fabrication by microfluidic tweezing. Langmuir, 24 (13), 6754-6758. (doi:10.1021/la8004823).

Record type: Article

Abstract

There is currently great interest in the development of lipid enclosed systems with complex geometrical arrangements that mimic cellular compartments. With biochemical functionalization, these soft matter devices can be used to probe deeper into life's transport dominated biochemical operations. In this paper, we present a novel tool for machining lipid nanotubules by microfluidic tweezing. A bilayer poly(dimethylsiloxane) (PDMS) device was designed with a lipid reservoir that was loaded by capillary action for lipid film deposition. The lipid reservoir is vertically separated from an upper flow for controlled material wetting and the formation of giant tubule bodies. Three fluidic paths are interfaced for introduction of the giant tubules into the high velocity center of a parabolic flow profile for exposure to hydrodynamic shear stresses. At local velocities approximating 2 mm s(-1), a 300-500 nm diameter jet of lipid material was tweezed from the giant tubule body and elongated with the flow. The high velocity flow provides uniform drag for the rapid and continuous fabrication of lipid nanotubules with tremendous axial ratios. Below a critical velocity, a remarkable shape transformation occurred and the projected lipid tubule grew until a constant 3.6 mu m diameter tubule was attained. These lipid tubules could be wired for the construction of advanced lifelike bioreactor systems.

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More information

e-pub ahead of print date: 27 May 2008
Published date: 1 July 2008
Additional Information: ISI Document Delivery No.: 318OI Times Cited: 7 Cited Reference Count: 38 West, Jonathan Manz, Andreas Dittrich, Petra S. Amer chemical soc Washington
Keywords: vesicle networks, electrophoretic transport, phospholipid membranes, surfactant liposomes, bilayers, tubules, device containers, extrusion
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 346440
URI: https://eprints.soton.ac.uk/id/eprint/346440
ISSN: 0743-7463
PURE UUID: c8117c98-a211-4887-b201-8cf010d764c2

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Date deposited: 28 Jan 2013 10:22
Last modified: 18 Jul 2017 05:04

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Contributors

Author: J. West
Author: A. Manz
Author: P. S. Dittrich

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