A microfluidic array with cellular valving for single cell co-culture
A microfluidic array with cellular valving for single cell co-culture
We present a highly parallel microfluidic approach for contacting single cell pairs. The approach combines a differential fluidic resistance trapping method with a novel cellular valving principle for homotypic and heterotypic single cell co-culturing. Differential fluidic resistance was used for sequential single cell arraying, with the adhesion and flattening of viable cells within the microstructured environment acting to produce valves in the open state. Reversal of the flow was used for the sequential single cell arraying of the second cell type. Plasma stencilling, along the linear path of least resistance, was required to confine the cells within the trap regions. Prime flow conditions with minimal shear stress were identified for highly efficient cell arraying (similar to 99%) and long term cell culture. Larger trap dimensions enabled the highest levels of cell pairing (similar to 70%). The single cell co-cultures were in close proximity for the formation of connexon structures and the study of contact modes of communication. The research further highlights the possibility of using the natural behaviour of cells as the working principle behind responsive microfluidic elements
hydrophobic, recovery, device, polydimethylsiloxane, electroporation, chip, flow
231-237
Frimat, Jean-Philippe
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Becker, Marco
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Chiang, Ya-Yu
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Marggraf, Ulrich
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Janasek, Dirk
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Hengstler, Jan G.
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Franzke, Joachim
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West, Jonathan
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2011
Frimat, Jean-Philippe
a15e6f17-c613-449d-88ca-18bb92f0834f
Becker, Marco
ea94c31a-c343-4fd4-a22f-1e80f4e44b7d
Chiang, Ya-Yu
0ab4fe9e-9a3e-4594-a13c-73f68c13b8b3
Marggraf, Ulrich
aadd4256-c0cd-4200-8335-235f891d80de
Janasek, Dirk
39a248d4-ac4a-4f07-9919-7bc1098cd715
Hengstler, Jan G.
8b1695f3-3506-400f-85ae-44b875949445
Franzke, Joachim
711b7072-1f6c-44e7-b33c-66a9c8304f77
West, Jonathan
f1c2e060-16c3-44c0-af70-242a1c58b968
Frimat, Jean-Philippe, Becker, Marco, Chiang, Ya-Yu, Marggraf, Ulrich, Janasek, Dirk, Hengstler, Jan G., Franzke, Joachim and West, Jonathan
(2011)
A microfluidic array with cellular valving for single cell co-culture.
Lab on a Chip, 11 (2), .
(doi:10.1039/C0LC00172D).
Abstract
We present a highly parallel microfluidic approach for contacting single cell pairs. The approach combines a differential fluidic resistance trapping method with a novel cellular valving principle for homotypic and heterotypic single cell co-culturing. Differential fluidic resistance was used for sequential single cell arraying, with the adhesion and flattening of viable cells within the microstructured environment acting to produce valves in the open state. Reversal of the flow was used for the sequential single cell arraying of the second cell type. Plasma stencilling, along the linear path of least resistance, was required to confine the cells within the trap regions. Prime flow conditions with minimal shear stress were identified for highly efficient cell arraying (similar to 99%) and long term cell culture. Larger trap dimensions enabled the highest levels of cell pairing (similar to 70%). The single cell co-cultures were in close proximity for the formation of connexon structures and the study of contact modes of communication. The research further highlights the possibility of using the natural behaviour of cells as the working principle behind responsive microfluidic elements
Text
Frimat_CoCulture_LOC2011.pdf
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Published date: 2011
Additional Information:
ISI Document Delivery No.: 697LI
Times Cited: 14
Cited Reference Count: 30
Frimat, Jean-Philippe Becker, Marco Chiang, Ya-Yu Marggraf, Ulrich Janasek, Dirk Hengstler, Jan G. Franzke, Joachim West, Jonathan
German Research Foundation (DFG) [WE3737/3-1]; Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen; Bundesministerium fur Bildung und Forschung
The authors are grateful to Maria Becker for SEM imaging, Joanna D. Stewart (IfADo) for support with statistical analysis, Wiebke Schormann (IfADo) for providing antibodies, and Sarah Waide and Susanne Funken for cell culture support. The research was financially supported by the German Research Foundation (DFG WE3737/3-1), the Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen and the Bundesministerium fur Bildung und Forschung.
Royal soc chemistry
Cambridge
Keywords:
hydrophobic, recovery, device, polydimethylsiloxane, electroporation, chip, flow
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 346446
URI: http://eprints.soton.ac.uk/id/eprint/346446
ISSN: 1473-0197
PURE UUID: da713f08-25fb-4665-8917-c0cdb3d8a5b9
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Date deposited: 04 Jan 2013 13:43
Last modified: 15 Mar 2024 03:43
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Contributors
Author:
Jean-Philippe Frimat
Author:
Marco Becker
Author:
Ya-Yu Chiang
Author:
Ulrich Marggraf
Author:
Dirk Janasek
Author:
Jan G. Hengstler
Author:
Joachim Franzke
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