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Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications
Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications
Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic characterization of nonfluorescent samples. Here we present a hyperspectral darkfield imaging system for spectral imaging of single nanoparticles over an area of 150 × 150 µm2 and at illumination intensities compatible with live cell imaging. The capabilities of the system are demonstrated using correlated transmission electron microscopy and single-particle optical studies of colloidal hollow gold nanoparticles. The potential of the system for characterizing the interactions between nanoparticles and cells has also been demonstrated. In this case, the spectral information proves a useful improvement to standard darkfield imaging as it enables differentiation between light scattered from nanoparticles and light scattered from other sources in the cellular environment. The combination of low illumination power and fast integration times makes the system highly suitable for nanoparticle tracking and spectroscopy in live-cell experiments.
1463-9076
4163-4168
Fairbairn, N.
3d290c5d-9ab3-4975-b008-308142880ba2
Christofidou, A.
1cdffad9-6782-4138-9b08-4991a50fb4b4
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Newman, T.A.
322290cb-2e9c-445d-a047-00b1bea39a25
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Fairbairn, N.
3d290c5d-9ab3-4975-b008-308142880ba2
Christofidou, A.
1cdffad9-6782-4138-9b08-4991a50fb4b4
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Newman, T.A.
322290cb-2e9c-445d-a047-00b1bea39a25
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9

Fairbairn, N., Christofidou, A., Kanaras, Antonios G., Newman, T.A. and Muskens, Otto L. (2013) Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications. Physical Chemistry Chemical Physics, 15, 4163-4168. (doi:10.1039/C2CP43162A). (PMID:23183927)

Record type: Article

Abstract

Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic characterization of nonfluorescent samples. Here we present a hyperspectral darkfield imaging system for spectral imaging of single nanoparticles over an area of 150 × 150 µm2 and at illumination intensities compatible with live cell imaging. The capabilities of the system are demonstrated using correlated transmission electron microscopy and single-particle optical studies of colloidal hollow gold nanoparticles. The potential of the system for characterizing the interactions between nanoparticles and cells has also been demonstrated. In this case, the spectral information proves a useful improvement to standard darkfield imaging as it enables differentiation between light scattered from nanoparticles and light scattered from other sources in the cellular environment. The combination of low illumination power and fast integration times makes the system highly suitable for nanoparticle tracking and spectroscopy in live-cell experiments.

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e-pub ahead of print date: 8 November 2012
Published date: 1 February 2013
Organisations: Quantum, Light & Matter Group

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Local EPrints ID: 346669
URI: https://eprints.soton.ac.uk/id/eprint/346669
ISSN: 1463-9076
PURE UUID: fc549f90-d6e7-4c35-8a75-b7cbb651f510
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706
ORCID for T.A. Newman: ORCID iD orcid.org/0000-0002-3727-9258
ORCID for Otto L. Muskens: ORCID iD orcid.org/0000-0003-0693-5504

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Date deposited: 07 Jan 2013 15:41
Last modified: 20 Jul 2019 01:17

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Contributors

Author: N. Fairbairn
Author: A. Christofidou
Author: T.A. Newman ORCID iD
Author: Otto L. Muskens ORCID iD

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