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The design of microfluidic affinity chromatography systems for the separation of bioanalytes

The design of microfluidic affinity chromatography systems for the separation of bioanalytes
The design of microfluidic affinity chromatography systems for the separation of bioanalytes
The analytical (numerical) design of planar microfluidic affinity chromatography devices, which consist of multiple separation lanes and multiple, different surface-immobilised receptor patterns in each lane, is described. The model is based on the analytical solution of the transport-reaction equations in microfluidic systems of low Gratz number and for injection of small analyte plugs. The results reveal a simple approach for the design of microfluidic affinity chromatography devices tailored to the separation of bioanalytes, where receptors with high binding affinity are available. These devices have been designed for bioanalytical applications in mind, most notably for the proteomics field; the results are illustrated with an example using β-Amyloid binding peptides.
affinity chromatography, microfluidic, mathematical modelling, separation, design strategy, ?-Amyloid binding peptides
1570-0232
163-171
Friedrich, Daniel
38fec5ed-5104-4d67-9354-2e064d0bdad2
Please, Colin P.
118dffe7-4b38-4787-a972-9feec535839e
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Friedrich, Daniel
38fec5ed-5104-4d67-9354-2e064d0bdad2
Please, Colin P.
118dffe7-4b38-4787-a972-9feec535839e
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Friedrich, Daniel, Please, Colin P. and Melvin, Tracy (2012) The design of microfluidic affinity chromatography systems for the separation of bioanalytes. Journal of Chromatography B, 910, 163-171. (doi:10.1016/j.jchromb.2012.09.041).

Record type: Article

Abstract

The analytical (numerical) design of planar microfluidic affinity chromatography devices, which consist of multiple separation lanes and multiple, different surface-immobilised receptor patterns in each lane, is described. The model is based on the analytical solution of the transport-reaction equations in microfluidic systems of low Gratz number and for injection of small analyte plugs. The results reveal a simple approach for the design of microfluidic affinity chromatography devices tailored to the separation of bioanalytes, where receptors with high binding affinity are available. These devices have been designed for bioanalytical applications in mind, most notably for the proteomics field; the results are illustrated with an example using β-Amyloid binding peptides.

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More information

Published date: 1 December 2012
Keywords: affinity chromatography, microfluidic, mathematical modelling, separation, design strategy, ?-Amyloid binding peptides
Organisations: Optoelectronics Research Centre, Mathematical Sciences

Identifiers

Local EPrints ID: 346691
URI: http://eprints.soton.ac.uk/id/eprint/346691
ISSN: 1570-0232
PURE UUID: 0840c262-0f28-4c50-bc7a-5076ca7e96e7

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Date deposited: 07 Jan 2013 14:27
Last modified: 07 Apr 2020 16:32

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Contributors

Author: Daniel Friedrich
Author: Colin P. Please
Author: Tracy Melvin

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