B-cell-T-cell activation and interaction in common variable immunodeficiency

Rezaei, Nima, Wing, James B., Aghamohammadi, Asghar, Carlring, Jennifer, Lees, Andrew, Asgarian-Omran, Hossein, Pourpak, Zahra, Sarrafnejad, Abdolfattah, Kardar, Gholam A, Shahrestani, Tahereh, Masoumi, Farimah, Zare, Ahad, Saghafi, Shiva, Sarrafzadeh, Shokouh, Foster, Rachel A., Heath, Andrew W. and Read, Robert C. (2010) B-cell-T-cell activation and interaction in common variable immunodeficiency. Human Immunology, 71 (4), 355-362. (doi:10.1016/j.humimm.2010.01.019). (PMID:20097245)

Abstract

Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by hypogammaglobulinemia and normal or low numbers of B cells, which predispose patients to recurrent infections. Peripheral blood mononuclear cells from 19 patients with CVID, and age- and sex-matched controls, were subjected to an in vitro assay of B-cell-T-cell activation and interaction, using anti-immunoglobulin (Ig)-D conjugated to dextran (alpha-delta-dex), as a polyclonal T independent type 2 antigen mimic, with and without anti-CD3/anti-CD28, as polyclonal T-cell stimuli. Stimulation of lymphocytes with either anti-CD3 or anti-CD3 plus anti-CD28 induced T-cell activation and proliferation in CVID patients who were similar to age- and sex-matched controls, but B cells of patients were significantly less activated when peripheral blood mononuclear cells were stimulated with polyclonal T-cell agonists alone. Comparison of CD86 expression in the patients with matched controls revealed that patients had low B-cell activation in response to T-cell stimuli (bystander T-cell help). In conclusion, this sample of CVID patients exhibits a defect of T-cell "help" to B cells, and/or B-cell response to T-cell help

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Contributors

Author: Robert C. Read

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