The University of Southampton
University of Southampton Institutional Repository

B-cell-T-cell activation and interaction in common variable immunodeficiency

B-cell-T-cell activation and interaction in common variable immunodeficiency
B-cell-T-cell activation and interaction in common variable immunodeficiency
Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by hypogammaglobulinemia and normal or low numbers of B cells, which predispose patients to recurrent infections. Peripheral blood mononuclear cells from 19 patients with CVID, and age- and sex-matched controls, were subjected to an in vitro assay of B-cell-T-cell activation and interaction, using anti-immunoglobulin (Ig)-D conjugated to dextran (alpha-delta-dex), as a polyclonal T independent type 2 antigen mimic, with and without anti-CD3/anti-CD28, as polyclonal T-cell stimuli. Stimulation of lymphocytes with either anti-CD3 or anti-CD3 plus anti-CD28 induced T-cell activation and proliferation in CVID patients who were similar to age- and sex-matched controls, but B cells of patients were significantly less activated when peripheral blood mononuclear cells were stimulated with polyclonal T-cell agonists alone. Comparison of CD86 expression in the patients with matched controls revealed that patients had low B-cell activation in response to T-cell stimuli (bystander T-cell help). In conclusion, this sample of CVID patients exhibits a defect of T-cell "help" to B cells, and/or B-cell response to T-cell help
0198-8859
355-362
Rezaei, Nima
4d41d46e-027a-4e28-bb0d-0e7faa7b9478
Wing, James B.
f1e7db81-a229-4386-87ea-f58dd0e8519a
Aghamohammadi, Asghar
90ffaeac-a13d-48cc-b3e6-b654bb6a6291
Carlring, Jennifer
6d5de399-1da9-45e6-83ac-b7735f806348
Lees, Andrew
dba4db52-3b05-4414-a801-e3255d068b67
Asgarian-Omran, Hossein
b429b837-8d1c-46ce-b6f7-7cddf2e95b2b
Pourpak, Zahra
d150a06c-2256-4469-850c-3c53badf9eb3
Sarrafnejad, Abdolfattah
fbf79649-aa63-481d-923c-17b876de8f93
Kardar, Gholam A
bfb4ba0b-bd2d-4acc-9609-7df3a0f82109
Shahrestani, Tahereh
7dd58171-63e6-4fa7-bfc4-b27e2332e2af
Masoumi, Farimah
f1a5e4d8-7f0e-4acf-86aa-9be4ae01f0ab
Zare, Ahad
dace35f2-570d-4674-bb0e-68187dbcd45d
Saghafi, Shiva
1c8c95bf-9b2d-4e0c-b556-ece9984d13e8
Sarrafzadeh, Shokouh
fc6f8300-675b-466d-8d66-2e3306a46390
Foster, Rachel A.
ae78b2b5-6fee-486e-ac49-b755d2d2314c
Heath, Andrew W.
303a3623-9848-4b85-add7-880752386f0d
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Rezaei, Nima
4d41d46e-027a-4e28-bb0d-0e7faa7b9478
Wing, James B.
f1e7db81-a229-4386-87ea-f58dd0e8519a
Aghamohammadi, Asghar
90ffaeac-a13d-48cc-b3e6-b654bb6a6291
Carlring, Jennifer
6d5de399-1da9-45e6-83ac-b7735f806348
Lees, Andrew
dba4db52-3b05-4414-a801-e3255d068b67
Asgarian-Omran, Hossein
b429b837-8d1c-46ce-b6f7-7cddf2e95b2b
Pourpak, Zahra
d150a06c-2256-4469-850c-3c53badf9eb3
Sarrafnejad, Abdolfattah
fbf79649-aa63-481d-923c-17b876de8f93
Kardar, Gholam A
bfb4ba0b-bd2d-4acc-9609-7df3a0f82109
Shahrestani, Tahereh
7dd58171-63e6-4fa7-bfc4-b27e2332e2af
Masoumi, Farimah
f1a5e4d8-7f0e-4acf-86aa-9be4ae01f0ab
Zare, Ahad
dace35f2-570d-4674-bb0e-68187dbcd45d
Saghafi, Shiva
1c8c95bf-9b2d-4e0c-b556-ece9984d13e8
Sarrafzadeh, Shokouh
fc6f8300-675b-466d-8d66-2e3306a46390
Foster, Rachel A.
ae78b2b5-6fee-486e-ac49-b755d2d2314c
Heath, Andrew W.
303a3623-9848-4b85-add7-880752386f0d
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Rezaei, Nima, Wing, James B., Aghamohammadi, Asghar, Carlring, Jennifer, Lees, Andrew, Asgarian-Omran, Hossein, Pourpak, Zahra, Sarrafnejad, Abdolfattah, Kardar, Gholam A, Shahrestani, Tahereh, Masoumi, Farimah, Zare, Ahad, Saghafi, Shiva, Sarrafzadeh, Shokouh, Foster, Rachel A., Heath, Andrew W. and Read, Robert C. (2010) B-cell-T-cell activation and interaction in common variable immunodeficiency. Human Immunology, 71 (4), 355-362. (doi:10.1016/j.humimm.2010.01.019). (PMID:20097245)

Record type: Article

Abstract

Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by hypogammaglobulinemia and normal or low numbers of B cells, which predispose patients to recurrent infections. Peripheral blood mononuclear cells from 19 patients with CVID, and age- and sex-matched controls, were subjected to an in vitro assay of B-cell-T-cell activation and interaction, using anti-immunoglobulin (Ig)-D conjugated to dextran (alpha-delta-dex), as a polyclonal T independent type 2 antigen mimic, with and without anti-CD3/anti-CD28, as polyclonal T-cell stimuli. Stimulation of lymphocytes with either anti-CD3 or anti-CD3 plus anti-CD28 induced T-cell activation and proliferation in CVID patients who were similar to age- and sex-matched controls, but B cells of patients were significantly less activated when peripheral blood mononuclear cells were stimulated with polyclonal T-cell agonists alone. Comparison of CD86 expression in the patients with matched controls revealed that patients had low B-cell activation in response to T-cell stimuli (bystander T-cell help). In conclusion, this sample of CVID patients exhibits a defect of T-cell "help" to B cells, and/or B-cell response to T-cell help

This record has no associated files available for download.

More information

Published date: April 2010
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 346797
URI: http://eprints.soton.ac.uk/id/eprint/346797
ISSN: 0198-8859
PURE UUID: 537bc264-6f0b-45bf-ad76-109b992128a5
ORCID for Robert C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 10 Jan 2013 09:37
Last modified: 15 Mar 2024 03:42

Export record

Altmetrics

Contributors

Author: Nima Rezaei
Author: James B. Wing
Author: Asghar Aghamohammadi
Author: Jennifer Carlring
Author: Andrew Lees
Author: Hossein Asgarian-Omran
Author: Zahra Pourpak
Author: Abdolfattah Sarrafnejad
Author: Gholam A Kardar
Author: Tahereh Shahrestani
Author: Farimah Masoumi
Author: Ahad Zare
Author: Shiva Saghafi
Author: Shokouh Sarrafzadeh
Author: Rachel A. Foster
Author: Andrew W. Heath
Author: Robert C. Read ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×