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Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
0934-9723
Almeida, C.
0a572983-c36b-40c3-bfc4-98e84b2bfda9
Azevedo, N. F.
cc6a32f1-5179-486a-84e3-5dc6f6854341
Bento, J. C.
28a2e70c-d0aa-45e4-9215-74a1c935f12f
Cerca, N.
bb17e55f-bad6-45da-be6b-ad93e903a2ab
Ramos, H.
5db8378f-4552-4a3f-9a5a-4bffd093b154
Vieira, M. J.
45deadf9-bf20-4e60-95e2-506226f0fed2
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Almeida, C.
0a572983-c36b-40c3-bfc4-98e84b2bfda9
Azevedo, N. F.
cc6a32f1-5179-486a-84e3-5dc6f6854341
Bento, J. C.
28a2e70c-d0aa-45e4-9215-74a1c935f12f
Cerca, N.
bb17e55f-bad6-45da-be6b-ad93e903a2ab
Ramos, H.
5db8378f-4552-4a3f-9a5a-4bffd093b154
Vieira, M. J.
45deadf9-bf20-4e60-95e2-506226f0fed2
Keevil, C. W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Almeida, C., Azevedo, N. F., Bento, J. C., Cerca, N., Ramos, H., Vieira, M. J. and Keevil, C. W. (2013) Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH. European Journal of Clinical Microbiology & Infectious Diseases. (doi:10.1007/s10096-012-1808-2).

Record type: Article

Abstract

We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.

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Published date: January 2013
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Organisations: Centre for Biological Sciences
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Identifiers

Local EPrints ID: 346942
URI: http://eprints.soton.ac.uk/id/eprint/346942
DOI: doi:10.1007/s10096-012-1808-2
ISSN: 0934-9723
PURE UUID: 1b6013fd-1e7b-4a82-8c12-7dcb305f7617
ORCID for C. W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 21 Jan 2013 14:39
Last modified: 15 Mar 2024 03:12

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Contributors

Author: C. Almeida
Author: N. F. Azevedo
Author: J. C. Bento
Author: N. Cerca
Author: H. Ramos
Author: M. J. Vieira
Author: C. W. Keevil ORCID iD

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