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Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour

Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour
Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour
Surface topography and surface chemistry can influence cell adhesion. This study evaluated how the combined effect of topography and chemistry can reduce cell spreading and proliferation. Silica nanoparticulate coatings ranging from 7, 14 and 21 nm in diameter were manufactured on glass substrates and chemically modified using organofunctional siloxanes bearing methyl (?CH3), sulphydryl (?SH), amine (?NH2), carboxyl (?COOH) and hydroxyl (?OH) terminal functional groups. Chemical modification of 7 nm silica induced changes in wettability with the advancing angle increasing for all modifications and the receding angle increasing for ?CH3, ?NH2 and ?SH group modifications. X-ray Photoelectron Spectroscopy (XPS) revealed that the surface chemistry was altered for all chemically modified surfaces confirming the modification step. The Field Emission Scanning Electron Microscopy (FESEM) demonstrated that the nanoparticulate coating remained intact after each chemical treatment. Optical microscopy from tissue culture experiments revealed changes in the morphology of the cells that could be attributed to the surface topography of the nanoparticulate coatings irrespective of the surface chemistry.
surface modification, nanoparticles, colloidal silica, cell interactions, organosilanes, surface analysis, cell morphology, cell proliferation, nanomaterials, surface topography, surface chemistry, cell behaviour, cell adhesion, silica coatings
1752-8933
320-338
Cousins, Brian G.
48a08cbb-55fb-4dd2-850c-2c513f5eb99d
Zekonyte, Jurgita
c40df725-5ce3-4692-b638-bbb4d847b5ea
Doherty, Patrick J.
7d919c14-6e3e-471f-b469-453004da879d
Garvey, Michael J.
504446bb-4698-4e20-be51-20d3dddcdaeb
Williams, Rachel L.
e9860b51-16e6-42a8-8f23-6b82d67b7771
Cousins, Brian G.
48a08cbb-55fb-4dd2-850c-2c513f5eb99d
Zekonyte, Jurgita
c40df725-5ce3-4692-b638-bbb4d847b5ea
Doherty, Patrick J.
7d919c14-6e3e-471f-b469-453004da879d
Garvey, Michael J.
504446bb-4698-4e20-be51-20d3dddcdaeb
Williams, Rachel L.
e9860b51-16e6-42a8-8f23-6b82d67b7771

Cousins, Brian G., Zekonyte, Jurgita, Doherty, Patrick J., Garvey, Michael J. and Williams, Rachel L. (2008) Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour. International Journal of Nano and Biomaterials, 1 (3), 320-338. (doi:10.1504/IJNBM.2008.016878).

Record type: Article

Abstract

Surface topography and surface chemistry can influence cell adhesion. This study evaluated how the combined effect of topography and chemistry can reduce cell spreading and proliferation. Silica nanoparticulate coatings ranging from 7, 14 and 21 nm in diameter were manufactured on glass substrates and chemically modified using organofunctional siloxanes bearing methyl (?CH3), sulphydryl (?SH), amine (?NH2), carboxyl (?COOH) and hydroxyl (?OH) terminal functional groups. Chemical modification of 7 nm silica induced changes in wettability with the advancing angle increasing for all modifications and the receding angle increasing for ?CH3, ?NH2 and ?SH group modifications. X-ray Photoelectron Spectroscopy (XPS) revealed that the surface chemistry was altered for all chemically modified surfaces confirming the modification step. The Field Emission Scanning Electron Microscopy (FESEM) demonstrated that the nanoparticulate coating remained intact after each chemical treatment. Optical microscopy from tissue culture experiments revealed changes in the morphology of the cells that could be attributed to the surface topography of the nanoparticulate coatings irrespective of the surface chemistry.

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More information

Published date: 2008
Keywords: surface modification, nanoparticles, colloidal silica, cell interactions, organosilanes, surface analysis, cell morphology, cell proliferation, nanomaterials, surface topography, surface chemistry, cell behaviour, cell adhesion, silica coatings
Organisations: nCATS Group

Identifiers

Local EPrints ID: 346990
URI: http://eprints.soton.ac.uk/id/eprint/346990
ISSN: 1752-8933
PURE UUID: 2aeaea8b-80eb-45e8-a49d-564ca023ba2b

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Date deposited: 07 May 2013 08:32
Last modified: 14 Mar 2024 12:44

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Contributors

Author: Brian G. Cousins
Author: Jurgita Zekonyte
Author: Patrick J. Doherty
Author: Michael J. Garvey
Author: Rachel L. Williams

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