The University of Southampton
University of Southampton Institutional Repository

Dynamic compressive strain influences chondrogenic gene expression in human periosteal cells: a case study

Dynamic compressive strain influences chondrogenic gene expression in human periosteal cells: a case study
Dynamic compressive strain influences chondrogenic gene expression in human periosteal cells: a case study
Physical stimuli play a crucial role in skeletogenesis and osteochondral repair and regeneration. Although the periosteum and periosteum-derived cells offer considerable therapeutic potential, the molecular mechanisms that control their differentiation are still not fully understood. As an initial case study, this work explores the hypothesis that dynamic compression might selectively enhance chondrogenic and/or osteogenic differentiation in human periosteal cells from two donors. Donor derived human periosteal cells were expanded in monolayer culture before seeding in 3% (w/v) agarose constructs. The ability of this in vitro culture model to support cell viability, chondrogenesis, and mechanotransduction was optimised. The time course of early chondrogenic differentiation was assessed by real time RT-PCR of mRNA expression levels for bone and cartilage specific gene markers. Intermittent dynamic compression (1 Hz, 15% strain) was applied to constructs, in the presence or absence of 10 ng/ml TGF-?3, for up to 4 days. The combined effect of TGF-?3 and compressive loading on the expression levels of the Sox-9, Runx-2, ALP, Collagen X, and collagen type I genes was donor dependent. A synergistic effect was noted only in donor two, with peak mRNA expression levels at 24 h, particularly Sox-9 which increased 59.0-fold. These findings suggest that the interactions between mechanical stimuli and TGF-? signalling may be an important mechanotransduction pathway for human periosteal cells and that, importantly, this cellular mechanosensitivity varies between donors.
periosteal-derived cells, chondrogenic differentiation, dynamic compression, mechanotransduction
1751-6161
72-81
Bonzani, I.C.
1d8b768d-0c3b-4736-bd68-b528aecbb9a2
Campbell, J.J.
42349934-cc6c-46da-84f8-ded60bb595a2
Knight, M.M.
eaff1c5c-595c-4b27-bb12-654dcfa0c1cb
Williams, A.
5906f63e-1283-4a30-9ec1-74d19d61b328
Lee, D.A.
fbbf7169-d08b-4deb-ae87-a2cbd97c58e7
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Stevens, M.M.
9d2f67c4-a1bc-46e1-8b64-2661bc86eb31
Bonzani, I.C.
1d8b768d-0c3b-4736-bd68-b528aecbb9a2
Campbell, J.J.
42349934-cc6c-46da-84f8-ded60bb595a2
Knight, M.M.
eaff1c5c-595c-4b27-bb12-654dcfa0c1cb
Williams, A.
5906f63e-1283-4a30-9ec1-74d19d61b328
Lee, D.A.
fbbf7169-d08b-4deb-ae87-a2cbd97c58e7
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Stevens, M.M.
9d2f67c4-a1bc-46e1-8b64-2661bc86eb31

Bonzani, I.C., Campbell, J.J., Knight, M.M., Williams, A., Lee, D.A., Bader, Dan L. and Stevens, M.M. (2012) Dynamic compressive strain influences chondrogenic gene expression in human periosteal cells: a case study. [in special issue: Special Issue on Tissue Engineering] Journal of the Mechanical Behavior of Biomedical Materials, 11, 72-81. (doi:10.1016/j.jmbbm.2011.06.015). (PMID:22658156)

Record type: Article

Abstract

Physical stimuli play a crucial role in skeletogenesis and osteochondral repair and regeneration. Although the periosteum and periosteum-derived cells offer considerable therapeutic potential, the molecular mechanisms that control their differentiation are still not fully understood. As an initial case study, this work explores the hypothesis that dynamic compression might selectively enhance chondrogenic and/or osteogenic differentiation in human periosteal cells from two donors. Donor derived human periosteal cells were expanded in monolayer culture before seeding in 3% (w/v) agarose constructs. The ability of this in vitro culture model to support cell viability, chondrogenesis, and mechanotransduction was optimised. The time course of early chondrogenic differentiation was assessed by real time RT-PCR of mRNA expression levels for bone and cartilage specific gene markers. Intermittent dynamic compression (1 Hz, 15% strain) was applied to constructs, in the presence or absence of 10 ng/ml TGF-?3, for up to 4 days. The combined effect of TGF-?3 and compressive loading on the expression levels of the Sox-9, Runx-2, ALP, Collagen X, and collagen type I genes was donor dependent. A synergistic effect was noted only in donor two, with peak mRNA expression levels at 24 h, particularly Sox-9 which increased 59.0-fold. These findings suggest that the interactions between mechanical stimuli and TGF-? signalling may be an important mechanotransduction pathway for human periosteal cells and that, importantly, this cellular mechanosensitivity varies between donors.

Full text not available from this repository.

More information

Published date: July 2012
Keywords: periosteal-derived cells, chondrogenic differentiation, dynamic compression, mechanotransduction
Organisations: Faculty of Health Sciences

Identifiers

Local EPrints ID: 347073
URI: https://eprints.soton.ac.uk/id/eprint/347073
ISSN: 1751-6161
PURE UUID: daab6aed-e50a-47ac-9248-94e73a6438b3
ORCID for Dan L. Bader: ORCID iD orcid.org/0000-0002-1208-3507

Catalogue record

Date deposited: 23 Jan 2013 09:48
Last modified: 06 Jun 2018 12:32

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×