Chemically amplified 19F–1H nuclear Overhauser effects
Chemically amplified 19F–1H nuclear Overhauser effects
Chemically induced dynamic nuclear polarisation (CIDNP) is explored as a source of nuclear hyperpolarisation in heteronuclear Overhauser effect experiments. A photochemical reaction proceeding through a radical pair intermediate is used to enhance 19F nuclear magnetisation in 3-fluorotyrosine by more than an order of magnitude with a corresponding increase in the amplitudes of 19F–1H cross-relaxation and cross-correlation effects. The reactions employed are cyclic and leave the sample chemically unchanged. The potential for enhancing the sensitivity of heteronuclear NOEs in 19F-labelled proteins is discussed.
nmr, cidnp, noe, fluorine, amino acid
1-7
Kuprov, I.
bb07f28a-5038-4524-8146-e3fc8344c065
Hore, P.J.
cad4561e-9571-4b49-b633-1c0bb470d144
May 2004
Kuprov, I.
bb07f28a-5038-4524-8146-e3fc8344c065
Hore, P.J.
cad4561e-9571-4b49-b633-1c0bb470d144
Kuprov, I. and Hore, P.J.
(2004)
Chemically amplified 19F–1H nuclear Overhauser effects.
Journal of Magnetic Resonance, 168 (1), .
(doi:10.1016/j.jmr.2004.01.011).
Abstract
Chemically induced dynamic nuclear polarisation (CIDNP) is explored as a source of nuclear hyperpolarisation in heteronuclear Overhauser effect experiments. A photochemical reaction proceeding through a radical pair intermediate is used to enhance 19F nuclear magnetisation in 3-fluorotyrosine by more than an order of magnitude with a corresponding increase in the amplitudes of 19F–1H cross-relaxation and cross-correlation effects. The reactions employed are cyclic and leave the sample chemically unchanged. The potential for enhancing the sensitivity of heteronuclear NOEs in 19F-labelled proteins is discussed.
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Published date: May 2004
Keywords:
nmr, cidnp, noe, fluorine, amino acid
Organisations:
Computational Systems Chemistry
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Local EPrints ID: 347397
URI: http://eprints.soton.ac.uk/id/eprint/347397
PURE UUID: f4a2d88d-e14a-48e5-94f3-9842f748f0c1
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Date deposited: 27 Feb 2013 12:29
Last modified: 15 Mar 2024 03:43
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Author:
P.J. Hore
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