The University of Southampton
University of Southampton Institutional Repository

Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver

Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver
Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver
Poor prenatal nutrition, acting through epigenetic processes, induces persistent changes in offspring phenotype. We investigated the effect of maternal fat intake on polyunsaturated fatty acid (PUFA) status and on the epigenetic regulation of Fads2, encoding ?6 desaturase (rate limiting in PUFA synthesis), in the adult offspring. Rats (n=6 per dietary group) were fed either 3.5% (w/w), 7% (w/w) or 21% (w/w) butter or fish oil (FO) from 14 days preconception until weaning. Offspring (n=6 males and females per dietary group) were fed 4% (w/w) soybean oil until postnatal day 77. 20:4n-6 and 22:6n-3 levels were lower in liver phosphatidylcholine (PC) and phosphatidylethanolamine and plasma PC (all P<.0001) in offspring of dams fed 21% than 3.5% or 7% fat regardless of type. Hepatic Fads2 expression related inversely to maternal dietary fat. Fads2 messenger RNA expression correlated negatively with methylation of CpGs at ?623, ?394, ?84 and ?76 bases relative to the transcription start site (all P<.005). Methylation of these CpGs was higher in offspring of dams fed 21% than 3.5% or 7% fat; FO higher than butter. Feeding adult female rats 7% fat reduced 20:4n-6 status in liver PC and Fads2 expression and increased methylation of CpGs ?623, ?394, ?84 and ?76 that reversed in animals switched from 7% to 4% fat diets. These findings suggest that fat exposure during development induces persistent changes, while adults exhibit a transient response, in hepatic PUFA status in offspring through epigenetic regulation of Fads2. Thus, epigenetic regulation of Fads2 may contribute to short- and long-term regulation of PUFA synthesis
0955-2863
1213-1220
Hoile, Samuel P.
9d7e9816-600d-45bd-ade2-dc7798bba730
Irvine, Nicola A.
ed181be8-0435-49b7-bbc9-ddf2249fd2aa
Kelsall, Christopher J.
f9a059aa-3add-451b-a595-899a64ba8123
Sibbons, Charlene
e613c761-bc82-4511-afd6-2d7e3cccc038
Feunteun, Aurelie
79885d89-e3d9-4d18-b99c-d09cf5f1a24c
Collister, Alex
0fc5d729-0c00-4818-ad43-6fe25c850288
Torrens, Christopher
15a35713-0651-4249-8227-5901e2cfcd22
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lillycrop, Karen A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Hoile, Samuel P.
9d7e9816-600d-45bd-ade2-dc7798bba730
Irvine, Nicola A.
ed181be8-0435-49b7-bbc9-ddf2249fd2aa
Kelsall, Christopher J.
f9a059aa-3add-451b-a595-899a64ba8123
Sibbons, Charlene
e613c761-bc82-4511-afd6-2d7e3cccc038
Feunteun, Aurelie
79885d89-e3d9-4d18-b99c-d09cf5f1a24c
Collister, Alex
0fc5d729-0c00-4818-ad43-6fe25c850288
Torrens, Christopher
15a35713-0651-4249-8227-5901e2cfcd22
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Hanson, Mark A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lillycrop, Karen A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159

Hoile, Samuel P., Irvine, Nicola A., Kelsall, Christopher J., Sibbons, Charlene, Feunteun, Aurelie, Collister, Alex, Torrens, Christopher, Calder, P.C., Hanson, Mark A., Lillycrop, Karen A. and Burdge, Graham C. (2013) Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and the epigenetic regulation of Fads2 in offspring liver. Journal of Nutritional Biochemistry, 24 (7), 1213-1220. (doi:10.1016/j.jnutbio.2012.09.005). (PMID:23107313)

Record type: Article

Abstract

Poor prenatal nutrition, acting through epigenetic processes, induces persistent changes in offspring phenotype. We investigated the effect of maternal fat intake on polyunsaturated fatty acid (PUFA) status and on the epigenetic regulation of Fads2, encoding ?6 desaturase (rate limiting in PUFA synthesis), in the adult offspring. Rats (n=6 per dietary group) were fed either 3.5% (w/w), 7% (w/w) or 21% (w/w) butter or fish oil (FO) from 14 days preconception until weaning. Offspring (n=6 males and females per dietary group) were fed 4% (w/w) soybean oil until postnatal day 77. 20:4n-6 and 22:6n-3 levels were lower in liver phosphatidylcholine (PC) and phosphatidylethanolamine and plasma PC (all P<.0001) in offspring of dams fed 21% than 3.5% or 7% fat regardless of type. Hepatic Fads2 expression related inversely to maternal dietary fat. Fads2 messenger RNA expression correlated negatively with methylation of CpGs at ?623, ?394, ?84 and ?76 bases relative to the transcription start site (all P<.005). Methylation of these CpGs was higher in offspring of dams fed 21% than 3.5% or 7% fat; FO higher than butter. Feeding adult female rats 7% fat reduced 20:4n-6 status in liver PC and Fads2 expression and increased methylation of CpGs ?623, ?394, ?84 and ?76 that reversed in animals switched from 7% to 4% fat diets. These findings suggest that fat exposure during development induces persistent changes, while adults exhibit a transient response, in hepatic PUFA status in offspring through epigenetic regulation of Fads2. Thus, epigenetic regulation of Fads2 may contribute to short- and long-term regulation of PUFA synthesis

This record has no associated files available for download.

More information

Published date: July 2013
Organisations: Human Development & Health, Centre for Biological Sciences

Identifiers

Local EPrints ID: 347562
URI: http://eprints.soton.ac.uk/id/eprint/347562
ISSN: 0955-2863
PURE UUID: ed9146ae-f7cb-46ae-acea-19fae9e6a6f4
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X
ORCID for Mark A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for Karen A. Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967

Catalogue record

Date deposited: 24 Jan 2013 14:32
Last modified: 02 Aug 2023 01:42

Export record

Altmetrics

Contributors

Author: Samuel P. Hoile
Author: Nicola A. Irvine
Author: Christopher J. Kelsall
Author: Charlene Sibbons
Author: Aurelie Feunteun
Author: Alex Collister
Author: Christopher Torrens
Author: P.C. Calder ORCID iD
Author: Mark A. Hanson ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×