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Comparison of venous and capillary differential leukocyte counts using a standard hematology analyzer and a novel microfluidic impedance cytometer

Comparison of venous and capillary differential leukocyte counts using a standard hematology analyzer and a novel microfluidic impedance cytometer
Comparison of venous and capillary differential leukocyte counts using a standard hematology analyzer and a novel microfluidic impedance cytometer
Capillary blood sampling has been identified as a potentially suitable technique for use in diagnostic testing of the full blood count (FBC) at the point-of-care (POC), for which a recent need has been highlighted. In this study we assess the accuracy of capillary blood counts and evaluate the potential of a miniaturized cytometer developed for POC testing. Differential leukocyte counts in the normal clinical range from fingerprick (capillary) and venous blood samples were measured and compared using a standard hematology analyzer. The accuracy of our novel microfluidic impedance cytometer (MIC) was then tested by comparing same-site measurements to those obtained with the standard analyzer. The concordance between measurements of fingerprick and venous blood samples using the standard hematology analyzer was high, with no clinically relevant differences observed between the mean differential leukocyte counts. Concordance data between the MIC and the standard analyzer on same-site measurements presented significantly lower leukocyte counts determined by the MIC. This systematic undercount was consistent across the measured (normal) concentration range, suggesting that an internal correction factor could be applied. Differential leukocyte counts obtained from fingerprick samples accurately reflect those from venous blood, which confirms the potential of capillary blood sampling for POC testing of the FBC. Furthermore, the MIC device demonstrated here presents a realistic technology for the future development of FBC and related tests for use at the site of patient care
1932-6203
e43702
Hollis, Veronica S
98fb298c-a05a-4ddc-9b4e-500762bfa007
Holloway, Judith A.
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Harris, Scott
19ea097b-df15-4f0f-be19-8ac42c190028
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
van Berkel, Cees
148b8dd1-6357-4308-81af-1ee11d287e99
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Hollis, Veronica S
98fb298c-a05a-4ddc-9b4e-500762bfa007
Holloway, Judith A.
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Harris, Scott
19ea097b-df15-4f0f-be19-8ac42c190028
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
van Berkel, Cees
148b8dd1-6357-4308-81af-1ee11d287e99
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Hollis, Veronica S, Holloway, Judith A., Harris, Scott, Spencer, Daniel, van Berkel, Cees and Morgan, Hywel (2012) Comparison of venous and capillary differential leukocyte counts using a standard hematology analyzer and a novel microfluidic impedance cytometer. PLoS ONE, 7 (9), e43702. (PMID:23028467)

Record type: Article

Abstract

Capillary blood sampling has been identified as a potentially suitable technique for use in diagnostic testing of the full blood count (FBC) at the point-of-care (POC), for which a recent need has been highlighted. In this study we assess the accuracy of capillary blood counts and evaluate the potential of a miniaturized cytometer developed for POC testing. Differential leukocyte counts in the normal clinical range from fingerprick (capillary) and venous blood samples were measured and compared using a standard hematology analyzer. The accuracy of our novel microfluidic impedance cytometer (MIC) was then tested by comparing same-site measurements to those obtained with the standard analyzer. The concordance between measurements of fingerprick and venous blood samples using the standard hematology analyzer was high, with no clinically relevant differences observed between the mean differential leukocyte counts. Concordance data between the MIC and the standard analyzer on same-site measurements presented significantly lower leukocyte counts determined by the MIC. This systematic undercount was consistent across the measured (normal) concentration range, suggesting that an internal correction factor could be applied. Differential leukocyte counts obtained from fingerprick samples accurately reflect those from venous blood, which confirms the potential of capillary blood sampling for POC testing of the FBC. Furthermore, the MIC device demonstrated here presents a realistic technology for the future development of FBC and related tests for use at the site of patient care

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Published date: 2012
Organisations: Nanoelectronics and Nanotechnology, Primary Care & Population Sciences, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 347881
URI: https://eprints.soton.ac.uk/id/eprint/347881
ISSN: 1932-6203
PURE UUID: 427f53b3-aeaf-4818-85be-49239f5a5c48
ORCID for Judith A. Holloway: ORCID iD orcid.org/0000-0002-2268-3071
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 01 Feb 2013 09:36
Last modified: 06 Jun 2018 12:52

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