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HPV detection and measurement of HPV-16, telomerase, and survivin transcripts in colposcopy clinic patients

HPV detection and measurement of HPV-16, telomerase, and survivin transcripts in colposcopy clinic patients
HPV detection and measurement of HPV-16, telomerase, and survivin transcripts in colposcopy clinic patients
Aims
To determine whether the detection of high risk human papillomavirus (HPV) types is more predictive for high grade CIN than the current cervical smear test, and whether the production and measurement of HPV type 16 (HPV-16) and cellular survivin and telomerase transcripts can be used to discriminate between cervical HPV infections that self cure and those that induce high grade lesions.

Methods
Three hundred and fifty four cervical smear samples from women attending the colposcopy clinic were tested by the polymerase chain reaction (PCR) for the presence of HPV. Transcripts for HPV-16 E6, E6*I, E6*II, E7, and L1 as well as cellular survivin, telomerase RNA component, and telomerase reverse transcriptase were measured using fluorogenic probe (Taqman) assays.

Results
Referral smear grades of severe or moderate showed greater positive predictive values for CIN 2/3 than did the detection of high or moderate risk HPV types. HPV-16 transcripts from E6, E6*I, E6*II, and E7 showed high predictive values for CIN 2/3, but low sensitivity. The telomerase RNA component was detected in 53 of 57 samples and telomerase reverse transcriptase was only detected in one sample, whereas survivin transcripts were detected in 40% of samples.

Conclusions
The detection of HPV-16 or cellular survivin or telomerase transcripts did not accurately predict the grade of CIN in the samples. The detection of HPV risk types correlated well with the grade of CIN; however, the referral grade smear was the most accurate predictor of the severity of the lesion. Of the 35 different HPV types detected, 18 are not included in the HPV hybrid capture II commercial test kit. The use of such kits would have missed HPV infection in 4.3% of clinic patients with CIN 2/3 lesions and 15.4% with CIN 0/1.
0021-9746
304-308
Lanham, S.A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Herbert, A.
710f7d17-429a-433e-8802-b96d78e4dead
Watt, P.J.
3ffa2cad-33c8-46bd-9aa1-74bfa9a37b4d
Lanham, S.A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
Herbert, A.
710f7d17-429a-433e-8802-b96d78e4dead
Watt, P.J.
3ffa2cad-33c8-46bd-9aa1-74bfa9a37b4d

Lanham, S.A., Herbert, A. and Watt, P.J. (2001) HPV detection and measurement of HPV-16, telomerase, and survivin transcripts in colposcopy clinic patients. Journal of Clinical Pathology, 54 (4), 304-308. (doi:10.1136/jcp.54.4.304). (PMID:11304848)

Record type: Article

Abstract

Aims
To determine whether the detection of high risk human papillomavirus (HPV) types is more predictive for high grade CIN than the current cervical smear test, and whether the production and measurement of HPV type 16 (HPV-16) and cellular survivin and telomerase transcripts can be used to discriminate between cervical HPV infections that self cure and those that induce high grade lesions.

Methods
Three hundred and fifty four cervical smear samples from women attending the colposcopy clinic were tested by the polymerase chain reaction (PCR) for the presence of HPV. Transcripts for HPV-16 E6, E6*I, E6*II, E7, and L1 as well as cellular survivin, telomerase RNA component, and telomerase reverse transcriptase were measured using fluorogenic probe (Taqman) assays.

Results
Referral smear grades of severe or moderate showed greater positive predictive values for CIN 2/3 than did the detection of high or moderate risk HPV types. HPV-16 transcripts from E6, E6*I, E6*II, and E7 showed high predictive values for CIN 2/3, but low sensitivity. The telomerase RNA component was detected in 53 of 57 samples and telomerase reverse transcriptase was only detected in one sample, whereas survivin transcripts were detected in 40% of samples.

Conclusions
The detection of HPV-16 or cellular survivin or telomerase transcripts did not accurately predict the grade of CIN in the samples. The detection of HPV risk types correlated well with the grade of CIN; however, the referral grade smear was the most accurate predictor of the severity of the lesion. Of the 35 different HPV types detected, 18 are not included in the HPV hybrid capture II commercial test kit. The use of such kits would have missed HPV infection in 4.3% of clinic patients with CIN 2/3 lesions and 15.4% with CIN 0/1.

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More information

Published date: April 2001
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 348217
URI: https://eprints.soton.ac.uk/id/eprint/348217
ISSN: 0021-9746
PURE UUID: 915a031f-6e16-4463-9737-ddaf36b10d42
ORCID for S.A. Lanham: ORCID iD orcid.org/0000-0002-4516-264X

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Date deposited: 05 Mar 2013 09:57
Last modified: 06 Jun 2018 13:02

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Contributors

Author: S.A. Lanham ORCID iD
Author: A. Herbert
Author: P.J. Watt

University divisions

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