The University of Southampton
University of Southampton Institutional Repository

Pilot study to assess the presence of Chlamydia trachomatis in urine from 18-30-year-old males using EIA/IF and PCR

Pilot study to assess the presence of Chlamydia trachomatis in urine from 18-30-year-old males using EIA/IF and PCR
Pilot study to assess the presence of Chlamydia trachomatis in urine from 18-30-year-old males using EIA/IF and PCR
Context. To increase detection, urine samples from young males could be opportunistically tested for Chlamydia trachomatis.
Objective. To determine C. trachomatis prevalence in urine, optimum specimen and compare sensitivity/feasibility of routine use of different testing methods.
Design. Group A, ‘sterile’ pyuria samples June 1998–January 1999, tested by enzyme immunoassay (EIA) and, if reactive, by immunofluorescence (IF). Subsequently batch-tested by polymerase chain reaction (PCR). Group B, consecutive urine samples October 1998–January 1999; batch-tested by PCR.
Setting. Microbiology laboratory.
Samples. From males aged 18–30 years; group A = 71, group B = 83.
Main outcome measures. Chlamydia trachomatis positive if EIA- and IF- or PCR-positive.
Results. Group A: 12 EIA/IF-positive; 9/12 and 15 EIAnegative samples PCR-positive. Group B: 11 PCR-positive; 8/11 showed ‘sterile’pyuria.
Conclusions.Opportunistic testing of urine from young men shows a significant number of C. trachomatis infections. ‘Sterile’ pyuria samples are optimal. EIA/IF are less sensitive than PCR but can be routinely performed and detect a significant proportion of cases.
1471-1893
36-37
Basarab, Adriana
f48d3d40-b406-4168-8705-cb9f3e33f64b
Browning, David
53b27290-2f14-44a8-9354-8a737782e549
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
O'Connell, Susan
0b91f1de-812d-40e6-b2c8-9f5ccacf9bbc
Basarab, Adriana
f48d3d40-b406-4168-8705-cb9f3e33f64b
Browning, David
53b27290-2f14-44a8-9354-8a737782e549
Lanham, Stuart A.
28fdbbef-e3b6-4fdf-bd0f-4968eeb614d6
O'Connell, Susan
0b91f1de-812d-40e6-b2c8-9f5ccacf9bbc

Basarab, Adriana, Browning, David, Lanham, Stuart A. and O'Connell, Susan (2002) Pilot study to assess the presence of Chlamydia trachomatis in urine from 18-30-year-old males using EIA/IF and PCR. Journal of Family Planning & Reproductive Health Care, 28 (1), 36-37. (doi:10.1783/147118902101195820). (PMID:16259815)

Record type: Article

Abstract

Context. To increase detection, urine samples from young males could be opportunistically tested for Chlamydia trachomatis.
Objective. To determine C. trachomatis prevalence in urine, optimum specimen and compare sensitivity/feasibility of routine use of different testing methods.
Design. Group A, ‘sterile’ pyuria samples June 1998–January 1999, tested by enzyme immunoassay (EIA) and, if reactive, by immunofluorescence (IF). Subsequently batch-tested by polymerase chain reaction (PCR). Group B, consecutive urine samples October 1998–January 1999; batch-tested by PCR.
Setting. Microbiology laboratory.
Samples. From males aged 18–30 years; group A = 71, group B = 83.
Main outcome measures. Chlamydia trachomatis positive if EIA- and IF- or PCR-positive.
Results. Group A: 12 EIA/IF-positive; 9/12 and 15 EIAnegative samples PCR-positive. Group B: 11 PCR-positive; 8/11 showed ‘sterile’pyuria.
Conclusions.Opportunistic testing of urine from young men shows a significant number of C. trachomatis infections. ‘Sterile’ pyuria samples are optimal. EIA/IF are less sensitive than PCR but can be routinely performed and detect a significant proportion of cases.

Full text not available from this repository.

More information

Published date: January 2002
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 348226
URI: http://eprints.soton.ac.uk/id/eprint/348226
ISSN: 1471-1893
PURE UUID: 1c85e477-53e2-4094-8f7c-451d9a0131fd
ORCID for Stuart A. Lanham: ORCID iD orcid.org/0000-0002-4516-264X

Catalogue record

Date deposited: 01 Mar 2013 10:42
Last modified: 20 Jul 2019 01:16

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×