Pilot study to assess the presence of Chlamydia trachomatis in urine from 18-30-year-old males using EIA/IF and PCR

Basarab, Adriana, Browning, David, Lanham, Stuart A. and O'Connell, Susan (2002) Pilot study to assess the presence of Chlamydia trachomatis in urine from 18-30-year-old males using EIA/IF and PCR. Journal of Family Planning & Reproductive Health Care, 28 (1), 36-37. (doi:10.1783/147118902101195820). (PMID:16259815)

Abstract

Context. To increase detection, urine samples from young males could be opportunistically tested for Chlamydia trachomatis.
Objective. To determine C. trachomatis prevalence in urine, optimum specimen and compare sensitivity/feasibility of routine use of different testing methods.
Design. Group A, ‘sterile’ pyuria samples June 1998–January 1999, tested by enzyme immunoassay (EIA) and, if reactive, by immunofluorescence (IF). Subsequently batch-tested by polymerase chain reaction (PCR). Group B, consecutive urine samples October 1998–January 1999; batch-tested by PCR.
Setting. Microbiology laboratory.
Samples. From males aged 18–30 years; group A = 71, group B = 83.
Main outcome measures. Chlamydia trachomatis positive if EIA- and IF- or PCR-positive.
Results. Group A: 12 EIA/IF-positive; 9/12 and 15 EIAnegative samples PCR-positive. Group B: 11 PCR-positive; 8/11 showed ‘sterile’pyuria.
Conclusions.Opportunistic testing of urine from young men shows a significant number of C. trachomatis infections. ‘Sterile’ pyuria samples are optimal. EIA/IF are less sensitive than PCR but can be routinely performed and detect a significant proportion of cases.

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Contributors

Author: Stuart A. Lanham

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