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Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean

Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean
Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean
Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of red autofluorescent picoplankton in surface waters of the South Atlantic Ocean. Using catalysed reporter deposition fluorescence in situ hybridization, virtually, all cells sorted from that group were affiliated with the Mamiellales clade II (84 ± 4%, division Chlorophyta) with a size of 1.6 ± 0.03 µm. Based on electron microscopy, the Mamiellales clade II–sorted cells have a simple morphology with apparently no scales, flagella or surface features. Their latitudinal distribution resembled the distribution of Synechococcus with very low concentrations in the surface waters of the Southern subtropical gyre (0.6–1.6 × 103 cells mL-1) and increased concentrations in the Southern temperate waters 8.3 × 103 cells mL-1. Identification of the flow cytometric group as Mamiellales clade II allowed us to characterize the morphology of these enigmatic uncultured picoplanktonic cells by electron microscopy and to determine their apparent preference for temperate rather than subtropical oceanic photic waters.
plastidic protists, planktonic picoeukaryotes, cell sorting, CARD-FISH, electron microscopy
664-671
Gómez-Pereira, Paola R.
fd05c8ca-71a8-4153-8644-c3ab129a6e8e
Kennaway, Gabrielle
579e590c-556a-46da-8547-ce372f8aed80
Fuchs, Bernhard M.
dfa49acc-93b0-4d04-87af-52ec11fa6b0e
Tarran, Glen A.
218a9233-a6ae-4d0c-a9ca-35ad0d5a35f7
Zubkov, Mikhail V.
b1dfb3a0-bcff-430c-9031-358a22b50743
Gómez-Pereira, Paola R.
fd05c8ca-71a8-4153-8644-c3ab129a6e8e
Kennaway, Gabrielle
579e590c-556a-46da-8547-ce372f8aed80
Fuchs, Bernhard M.
dfa49acc-93b0-4d04-87af-52ec11fa6b0e
Tarran, Glen A.
218a9233-a6ae-4d0c-a9ca-35ad0d5a35f7
Zubkov, Mikhail V.
b1dfb3a0-bcff-430c-9031-358a22b50743

Gómez-Pereira, Paola R., Kennaway, Gabrielle, Fuchs, Bernhard M., Tarran, Glen A. and Zubkov, Mikhail V. (2013) Flow cytometric identification of Mamiellales clade II in the Southern Atlantic Ocean. FEMS Microbiology Ecology, 83 (3), 664-671. (doi:10.1111/1574-6941.12023).

Record type: Article

Abstract

Flow cytometric sorting, based on cellular optical properties and macromolecule content, has been successfully employed to taxonomically affiliate bacterioplankton. However, this approach has not been much used for eukaryotic plankton. To redress this imbalance, we identified a conspicuous group of red autofluorescent picoplankton in surface waters of the South Atlantic Ocean. Using catalysed reporter deposition fluorescence in situ hybridization, virtually, all cells sorted from that group were affiliated with the Mamiellales clade II (84 ± 4%, division Chlorophyta) with a size of 1.6 ± 0.03 µm. Based on electron microscopy, the Mamiellales clade II–sorted cells have a simple morphology with apparently no scales, flagella or surface features. Their latitudinal distribution resembled the distribution of Synechococcus with very low concentrations in the surface waters of the Southern subtropical gyre (0.6–1.6 × 103 cells mL-1) and increased concentrations in the Southern temperate waters 8.3 × 103 cells mL-1. Identification of the flow cytometric group as Mamiellales clade II allowed us to characterize the morphology of these enigmatic uncultured picoplanktonic cells by electron microscopy and to determine their apparent preference for temperate rather than subtropical oceanic photic waters.

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More information

Published date: March 2013
Keywords: plastidic protists, planktonic picoeukaryotes, cell sorting, CARD-FISH, electron microscopy
Organisations: Marine Biogeochemistry

Identifiers

Local EPrints ID: 348336
URI: http://eprints.soton.ac.uk/id/eprint/348336
PURE UUID: 0120abd7-aa50-44b6-bc3a-71e28791e61a

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Date deposited: 12 Feb 2013 11:47
Last modified: 14 Mar 2024 12:58

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Contributors

Author: Paola R. Gómez-Pereira
Author: Gabrielle Kennaway
Author: Bernhard M. Fuchs
Author: Glen A. Tarran
Author: Mikhail V. Zubkov

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