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The use of commercial products for the detection of Cryptosporidium oocysts throughout the food chain

The use of commercial products for the detection of Cryptosporidium oocysts throughout the food chain
The use of commercial products for the detection of Cryptosporidium oocysts throughout the food chain
Cryptosporidium parvum is an obligate parasite that has been shown to infect a range of mammalian species including humans, cattle, deer and mice. This parasite infects the gastro-intestinal tract producing watery diarrhoea containing parasitic eggs called oocysts. These shed oocysts are the environmental form of the parasites life cycle. The oocysts are readily found surviving for long periods in shed faeces, sewage sludge applied to land, wastewater, and surface water. This means that the processing of surface water to produce potable water for human consumption has to be stringent and closely monitored for breakthrough of viable Cryptosporidium oocysts. This potential for infecting the public was emphasised by a number of high profile water-borne outbreaks. The largest outbreak occurred in Milwaukee, Wisconsin, USA where over 400,000 residents were exposed to this parasite.

Due to these outbreaks and general rise in the numbers of cases being reported each year there has been a great deal of activity in the commercial sector to develop suitable detection systems to isolate, purify, detect and enumerate Cryptosporidium oocysts from key samples in the food chain.

These key samples include:
Potable water
Veterinary & Human faecal samples
Food ingredients & Food samples

Of these samples Potable water and faecal samples have been targeted for detection product development as these represent the largest markets for testing at present. This paper aims to describe the types of detection systems that have been developed, primarily for Potable water and faecal samples, to improve and enhance the methods for detection of Cryptosporidium oocysts in this wide range of sample types.
84-93
Teagasc
Clark, S.A.
daab7211-14eb-4d48-a898-6daaa5d767f6
Watkins, J.
bdd2fba0-01db-49ed-8efa-405c0b3be913
Wilks, S.A.
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Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Clark, S.A.
daab7211-14eb-4d48-a898-6daaa5d767f6
Watkins, J.
bdd2fba0-01db-49ed-8efa-405c0b3be913
Wilks, S.A.
86c1f41a-12b3-451c-9245-b1a21775e993
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb

Clark, S.A., Watkins, J., Wilks, S.A. and Keevil, C.W. (2003) The use of commercial products for the detection of Cryptosporidium oocysts throughout the food chain. In Cryptosporidium parvum in food and water. Teagasc. pp. 84-93 .

Record type: Conference or Workshop Item (Paper)

Abstract

Cryptosporidium parvum is an obligate parasite that has been shown to infect a range of mammalian species including humans, cattle, deer and mice. This parasite infects the gastro-intestinal tract producing watery diarrhoea containing parasitic eggs called oocysts. These shed oocysts are the environmental form of the parasites life cycle. The oocysts are readily found surviving for long periods in shed faeces, sewage sludge applied to land, wastewater, and surface water. This means that the processing of surface water to produce potable water for human consumption has to be stringent and closely monitored for breakthrough of viable Cryptosporidium oocysts. This potential for infecting the public was emphasised by a number of high profile water-borne outbreaks. The largest outbreak occurred in Milwaukee, Wisconsin, USA where over 400,000 residents were exposed to this parasite.

Due to these outbreaks and general rise in the numbers of cases being reported each year there has been a great deal of activity in the commercial sector to develop suitable detection systems to isolate, purify, detect and enumerate Cryptosporidium oocysts from key samples in the food chain.

These key samples include:
Potable water
Veterinary & Human faecal samples
Food ingredients & Food samples

Of these samples Potable water and faecal samples have been targeted for detection product development as these represent the largest markets for testing at present. This paper aims to describe the types of detection systems that have been developed, primarily for Potable water and faecal samples, to improve and enhance the methods for detection of Cryptosporidium oocysts in this wide range of sample types.

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More information

Published date: 1 January 2003
Venue - Dates: Cryptosporidium parvum in Food and Water, 2003-01-01
Organisations: Environmental

Identifiers

Local EPrints ID: 348397
URI: http://eprints.soton.ac.uk/id/eprint/348397
PURE UUID: 6bb46176-d459-4376-aed5-a4d3d163b929
ORCID for S.A. Wilks: ORCID iD orcid.org/0000-0002-4134-9415
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 05 Mar 2013 10:13
Last modified: 23 Jul 2022 01:49

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Contributors

Author: S.A. Clark
Author: J. Watkins
Author: S.A. Wilks ORCID iD
Author: C.W. Keevil ORCID iD

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