Nanotopographical cues augment mesenchymal differentiation of human embryonic stem cells
Nanotopographical cues augment mesenchymal differentiation of human embryonic stem cells
The production of bone-forming osteogenic cells for research purposes or transplantation therapies remains a significant challenge. Using planar polycarbonate substrates lacking in topographical cues and substrates displaying a nanotopographical pattern, mesenchymal differentiation of human embryonic stem cells is directed in the absence of chemical factors and without induction of differentiation by embryoid body formation. Cells incubated on nanotopographical substrates show enhanced expression of mesenchymal or stromal markers and expression of early osteogenic progenitors at levels above those detected in cells on planar substrates in the same basal media. Evidence of epithelial-to-mesenchymal transition during substrate differentiation and DNA methylation changes akin to chemical induction are also observed. These studies provide a suitable approach to overcome regenerative medical challenges and describe a defined, reproducible platform for human embryonic stem cell differentiation
human embryonic stem cells, nanotopography, differentiation, mesoderms, mesenchymal differentiation
2140-2151
Kingham, Emma Jayne
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White, Kate
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Gadegaard, Nikolaj
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Dalby, Matthew J.
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Oreffo, Richard O.C.
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June 2013
Kingham, Emma Jayne
05ad1291-8428-411a-9202-2577e7ff555c
White, Kate
6d488c09-d594-4791-be13-9844f8ded26a
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kingham, Emma Jayne, White, Kate, Gadegaard, Nikolaj, Dalby, Matthew J. and Oreffo, Richard O.C.
(2013)
Nanotopographical cues augment mesenchymal differentiation of human embryonic stem cells.
Small, 9 (12), .
(doi:10.1002/smll.201202340).
(PMID:23362187)
Abstract
The production of bone-forming osteogenic cells for research purposes or transplantation therapies remains a significant challenge. Using planar polycarbonate substrates lacking in topographical cues and substrates displaying a nanotopographical pattern, mesenchymal differentiation of human embryonic stem cells is directed in the absence of chemical factors and without induction of differentiation by embryoid body formation. Cells incubated on nanotopographical substrates show enhanced expression of mesenchymal or stromal markers and expression of early osteogenic progenitors at levels above those detected in cells on planar substrates in the same basal media. Evidence of epithelial-to-mesenchymal transition during substrate differentiation and DNA methylation changes akin to chemical induction are also observed. These studies provide a suitable approach to overcome regenerative medical challenges and describe a defined, reproducible platform for human embryonic stem cell differentiation
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Kingham et al Small 2013.pdf
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Published date: June 2013
Keywords:
human embryonic stem cells, nanotopography, differentiation, mesoderms, mesenchymal differentiation
Organisations:
Human Development & Health
Identifiers
Local EPrints ID: 348662
URI: http://eprints.soton.ac.uk/id/eprint/348662
ISSN: 1613-6810
PURE UUID: c6e75845-5367-4f59-9f96-73e9307f2074
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Date deposited: 18 Feb 2013 11:18
Last modified: 15 Mar 2024 03:04
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Contributors
Author:
Emma Jayne Kingham
Author:
Kate White
Author:
Nikolaj Gadegaard
Author:
Matthew J. Dalby
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