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Assessing the potential of colony morphology for dissecting the CFU-F population from human bone marrow stromal cells

Assessing the potential of colony morphology for dissecting the CFU-F population from human bone marrow stromal cells
Assessing the potential of colony morphology for dissecting the CFU-F population from human bone marrow stromal cells
Mesenchymal stem cells (MSCs) provide an ideal cell source for bone tissue engineering strategies. However, bone marrow stromal cell (BMSC) populations that contain MSCs are highly heterogeneous expressing a wide variety of proliferative and differentiation potentials. Current MSC isolation methods employing magnetic-activated and fluorescent-activated cell sorting can be expensive and time consuming and, in the absence of specific MSC markers, fail to generate homogeneous populations. We have investigated the potential of various colony morphology descriptors to provide correlations with cell growth potential. Density-independent colony forming unit-fibroblastic (CFU-F) capacity is a MSC prerequisite and resultant colonies display an array of shapes and sizes that might be representative of cell function. Parent colonies were initially categorised according to their diameter and cell density and grouped before passage for the subsequent assessment of progeny colonies. Whereas significant morphological differences between distinct parent populations indicated a correlation with immunophenotype, enhanced CFU-F capacity was not observed when individual colonies were isolated according to these morphological parameters. Colony circularity, an alternative morphological measure, displayed a strong correlation with subsequent cell growth potential. The current study indicates the potential of morphological descriptors for predicting cell growth rate and suggests new directions for research into dissection of human BMSC CFU-F populations.
0302-766X
Gothard, D.
7ff8059e-6541-4f0f-9cdd-4f6edf0f6338
Dawson, J.I.
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Gothard, D.
7ff8059e-6541-4f0f-9cdd-4f6edf0f6338
Dawson, J.I.
b220fe76-498d-47be-9995-92da6c289cf3
Oreffo, R.O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Gothard, D., Dawson, J.I. and Oreffo, R.O.C. (2013) Assessing the potential of colony morphology for dissecting the CFU-F population from human bone marrow stromal cells. Cell and Tissue Research. (doi:10.1007/s00441-013-1564-3). (PMID:23397425)

Record type: Article

Abstract

Mesenchymal stem cells (MSCs) provide an ideal cell source for bone tissue engineering strategies. However, bone marrow stromal cell (BMSC) populations that contain MSCs are highly heterogeneous expressing a wide variety of proliferative and differentiation potentials. Current MSC isolation methods employing magnetic-activated and fluorescent-activated cell sorting can be expensive and time consuming and, in the absence of specific MSC markers, fail to generate homogeneous populations. We have investigated the potential of various colony morphology descriptors to provide correlations with cell growth potential. Density-independent colony forming unit-fibroblastic (CFU-F) capacity is a MSC prerequisite and resultant colonies display an array of shapes and sizes that might be representative of cell function. Parent colonies were initially categorised according to their diameter and cell density and grouped before passage for the subsequent assessment of progeny colonies. Whereas significant morphological differences between distinct parent populations indicated a correlation with immunophenotype, enhanced CFU-F capacity was not observed when individual colonies were isolated according to these morphological parameters. Colony circularity, an alternative morphological measure, displayed a strong correlation with subsequent cell growth potential. The current study indicates the potential of morphological descriptors for predicting cell growth rate and suggests new directions for research into dissection of human BMSC CFU-F populations.

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e-pub ahead of print date: 9 February 2013
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 348663
URI: http://eprints.soton.ac.uk/id/eprint/348663
ISSN: 0302-766X
PURE UUID: 4fb5627a-c775-4809-a5c4-28aa61105f18
ORCID for J.I. Dawson: ORCID iD orcid.org/0000-0002-6712-0598
ORCID for R.O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 15 Feb 2013 12:54
Last modified: 02 Aug 2023 01:51

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Contributors

Author: D. Gothard
Author: J.I. Dawson ORCID iD
Author: R.O.C. Oreffo ORCID iD

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