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Prostate cells exposed to lycopene in vitro liberate lycopene-enriched exosomes

Prostate cells exposed to lycopene in vitro liberate lycopene-enriched exosomes
Prostate cells exposed to lycopene in vitro liberate lycopene-enriched exosomes
OBJECTIVES: To investigate whether cellular exosomes liberated by prostatic cell lines in culture might be acting as the transport vehicles for the dietary antioxidant lycopene, known to be sequestered in the prostate gland and to reduce the risk of developing benign prostatic hyperplasia (BPH) and prostate cancer; its subsequent secretion into seminal plasma also confers protection to spermatozoa against oxidative free-radical damage.

MATERIALS AND METHODS: Using benign and malignant human prostatic cell culture models, we assessed the role that their exosomes (the putative in vitro analogues of prostasomes) might have in the transport of lycopene.

RESULTS: Cells exposed to lycopene in vitro accumulated the molecule and secrete lycopene-enriched exosomes. This continued after the lycopene exposure was stopped. Extraction of lycopene from the exosomes, followed by high-performance liquid chromatography, confirmed nanogram quantities of lycopene per milligram of exosomal protein. Packaging into exosomes for export resulted in reduced degradation of this labile antioxidant, and therefore maximized the effectiveness of delivery to the sites of action.

CONCLUSION: These results support the likelihood that these organelles act as the transport vehicles for this important lipophilic agent known to have a role in the chemoprevention of various urological pathologies such as BPH, prostate cancer and male infertility.
in vitro, lycopene, prostate, infertility, exosomes, prostasomes
1464-4096
907-911
Goyal, Anuj
e3236f2e-22ea-4367-84e4-b2d51f06129e
Delves, George H.
e24020b5-6bf1-49b5-96ea-860b7886d8fa
Chopra, Mridula
9ac7d722-f547-4303-a966-b9dfe7e50387
Lwaleed, Bashir A.
0b5ea0b9-767b-4b47-a5dd-7798e6300181
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5
Goyal, Anuj
e3236f2e-22ea-4367-84e4-b2d51f06129e
Delves, George H.
e24020b5-6bf1-49b5-96ea-860b7886d8fa
Chopra, Mridula
9ac7d722-f547-4303-a966-b9dfe7e50387
Lwaleed, Bashir A.
0b5ea0b9-767b-4b47-a5dd-7798e6300181
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5

Goyal, Anuj, Delves, George H., Chopra, Mridula, Lwaleed, Bashir A. and Cooper, Alan J. (2006) Prostate cells exposed to lycopene in vitro liberate lycopene-enriched exosomes. BJU International, 98 (4), 907-911. (doi:10.1111/j.1464-410X.2006.06434.x). (PMID:16978292)

Record type: Article

Abstract

OBJECTIVES: To investigate whether cellular exosomes liberated by prostatic cell lines in culture might be acting as the transport vehicles for the dietary antioxidant lycopene, known to be sequestered in the prostate gland and to reduce the risk of developing benign prostatic hyperplasia (BPH) and prostate cancer; its subsequent secretion into seminal plasma also confers protection to spermatozoa against oxidative free-radical damage.

MATERIALS AND METHODS: Using benign and malignant human prostatic cell culture models, we assessed the role that their exosomes (the putative in vitro analogues of prostasomes) might have in the transport of lycopene.

RESULTS: Cells exposed to lycopene in vitro accumulated the molecule and secrete lycopene-enriched exosomes. This continued after the lycopene exposure was stopped. Extraction of lycopene from the exosomes, followed by high-performance liquid chromatography, confirmed nanogram quantities of lycopene per milligram of exosomal protein. Packaging into exosomes for export resulted in reduced degradation of this labile antioxidant, and therefore maximized the effectiveness of delivery to the sites of action.

CONCLUSION: These results support the likelihood that these organelles act as the transport vehicles for this important lipophilic agent known to have a role in the chemoprevention of various urological pathologies such as BPH, prostate cancer and male infertility.

Full text not available from this repository.

More information

e-pub ahead of print date: 5 September 2006
Published date: October 2006
Keywords: in vitro, lycopene, prostate, infertility, exosomes, prostasomes
Organisations: Faculty of Health Sciences

Identifiers

Local EPrints ID: 348736
URI: http://eprints.soton.ac.uk/id/eprint/348736
ISSN: 1464-4096
PURE UUID: 02d3aa09-6960-4419-accf-5b27433dd5e9

Catalogue record

Date deposited: 18 Feb 2013 16:45
Last modified: 16 Jul 2019 21:43

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